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Russian Journal of Bioorganic Chemistry

, Volume 44, Issue 1, pp 112–122 | Cite as

Dopamine Pretreatment Protects Offspring Rats from LPS-Induced Hypertension and Kidney Damage by Inhibiting NLRP3 Activation in Kidney

  • X. Q. Hao
  • C. F. Huang
  • F. Liang
  • W. Deng
  • Y. H. Ou
  • X. Zhang
  • L. Ding
  • D. Wang
  • S. T. Wang
Article
  • 11 Downloads

Abstract

This study was designed to explore the role of dopamine (DA) and NLRP3 in the mechanism of hypertension and renal impairment induced by prenatal exposure to lipopolysaccharide (LPS). Thirty pregnant rats were randomly divided into three groups, including control, LPS, and DA + LPS groups. Renal morphology changes were observed under microscope. The serum IL-1β and IL-18 concentration, mRNA, and protein expressions of NLRP3, ASC, and CASP1 in kidney of three-month old offspring rats were measured. Hematoxylin–eosin staining showed that the glomerular diameter decreased and cysts increased partly, glomerular balloons were adhered partly, there were moderate-to-severe hyperplasia dispersedly distributed in glomerular mesangial matrix in rats of LPS group. Serum IL-1β and IL-18 concentration in LPS group increased compared with control group and DA + LPS group. The average arterial pressure of offspring rats in LPS group increased significantly compared with control group and DA + LPS group. NLRP3 mRNA level in the LPS group increased significantly compared with the control group and the DA + LPS group in both male and female rats; NLRP3, ASC, and CASP1 protein expression in both renal cortex and renal medulla of LPS group increased significantly compared with control group and DA + LPS group in female rats. NLRP3 protein expression in LPS group increased significantly compared with control group and DA + LPS group in male rats. DA pretreatment exerts a protective effect on LPS-induced renal injury and hypertension in offspring rats, and the mechanism might be through inhibiting NLRP3 activation.

Keywords

prenatal stress lipopolysaccharide hypertension dopamine NLRP3 inflammation 

Abbreviation

DA

dopamine

LPS

lipopolysaccharide

EH

essential hypertension

NLRP3

nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3

ASC

apoptosis-associated speck-like protein containing a caspase recruitment domain

CASP1

caspase-1

PAMPs

pathogen-associated molecular patterns

DAMPs

danger-associated molecular patterns

IL

interleukin

DRD1

dopamine 1 receptor

BUN

blood urea nitrogen

UA

uric acid

Cre

creatinine

ELISA

enzyme-linked immunosorbent assay

HE

hematoxylin and eosin

TLR4

Toll-like receptor 4

AKI

acute kidney injury

IRI

ischemia/reperfusion injury

ATN

acute tubular necrosis

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • X. Q. Hao
    • 1
  • C. F. Huang
    • 1
  • F. Liang
    • 1
  • W. Deng
    • 1
  • Y. H. Ou
    • 1
  • X. Zhang
    • 1
  • L. Ding
    • 1
  • D. Wang
    • 1
  • S. T. Wang
    • 1
  1. 1.College of Animal Science and TechnologyHenan University of Science and TechnologyLuoyangChina

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