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Russian Journal of Bioorganic Chemistry

, Volume 44, Issue 1, pp 80–89 | Cite as

Study of the Carbohydrate Specificity of Antibodies Against Aspergillus fumigatus Using the Library of Synthetic Mycoantigens

  • V. B. Krylov
  • M. I. Petruk
  • I. V. Grigoryev
  • Y. S. Lebedin
  • N. I. Glushko
  • E. V. Khaldeeva
  • D. A. Argunov
  • E. A. Khatuntseva
  • M. V. Toplishek
  • B. S. Komarova
  • A. A. Karelin
  • O. N. Yudina
  • V. M. Menshov
  • D. V. Yashunskii
  • Y. E. Tsvetkov
  • N. E. Nifantiev
Article
  • 27 Downloads

Abstract

The carbohydrate specificity of antibodies obtained by immunizing laboratory animals with immunogens prepared from the Aspergillus fumigatus cell wall was analyzed using the library of biotinylated synthetic oligosaccharides. It has been shown that the main part of antipolysaccharide antibodies recognizes galactomannan in the studied immunogens with preference to its fragments containing more than two β-(1→5)-linked galactofuranosyl units. The data obtained can form the basis for the development of enzyme immunoassay for the detection of this dangerous fungal pathogen.

Keywords

Aspergillus fumigatus aspergillosis antigen antibody galactomannan diagnosticum 

Abbreviation

MWCO

molecular weight cut-off

EDAC

1-ethyl-3-(3-dimethylaminopropyl)carbodiimide

PBS

phosphate-buffered saline.

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. B. Krylov
    • 1
  • M. I. Petruk
    • 1
  • I. V. Grigoryev
    • 2
  • Y. S. Lebedin
    • 2
  • N. I. Glushko
    • 3
  • E. V. Khaldeeva
    • 3
  • D. A. Argunov
    • 1
  • E. A. Khatuntseva
    • 1
  • M. V. Toplishek
    • 1
  • B. S. Komarova
    • 1
  • A. A. Karelin
    • 1
  • O. N. Yudina
    • 1
  • V. M. Menshov
    • 1
  • D. V. Yashunskii
    • 1
  • Y. E. Tsvetkov
    • 1
  • N. E. Nifantiev
    • 1
  1. 1.Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.XEMA Company LimitedMoscowRussia
  3. 3.Kazan Scientific Research Institute of Epidemiology and MicrobiologyKazanRussia

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