Abstract
26-Hydroxyderivatives of 6-deoxo-24-epicastasterone and 6-deoxo-28-norcastasterone were synthesized for preparation of the corresponding haptens and conjugates with horseradish peroxidase and bovine serum albumin. These preparations were further applied to a production of antisera. Test-systems for the enzyme immunoassay of phytohormones of the 6-deoxobrassinosteroid group were elaborated.
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Abbreviations
- BS:
-
brassinosteroid
- 6-DONC:
-
6-deoxo-28-norcastastrone
- 6-DOEC:
-
6-deoxo-24-epicastasterone
- BSA:
-
bovine serum albumin
- HP:
-
horseradish peroxidase
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Original Russian Text © R.P. Litvinovskaya, A.L. Sauchuk, K.G. Kazharnovich, A.G. Pradko, T.V. Mirantsova, V.N. Zhabinskii, V.A. Khripach, 2017, published in Bioorganicheskaya Khimiya, 2017, Vol. 43, No. 3, pp. 284–300.
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Litvinovskaya, R.P., Sauchuk, A.L., Kazharnovich, K.G. et al. Test-systems for immunoenzymatic determination of 6-deoxobrassinosteroids. Russ J Bioorg Chem 43, 286–301 (2017). https://doi.org/10.1134/S1068162017030098
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DOI: https://doi.org/10.1134/S1068162017030098