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Investigation of the complex antibiotic INA-5812


A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study.

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colony-forming unit


reversedphase high performance liquid chromatography




high-resolution electrospray ionization mass spectrometry


matrix-assisted laser desorption/ionization time-of-flight mass spectrometry


trifluoroacetic acid


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Correspondence to A. P. Tyurin.

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Original Russian Text © O.A. Lapchinskaya, G.S. Katrukha, E.G. Gladkikh, V.V. Kulyaeva, P.V. Coodan, A.P. Topolyan, V.A. Alferova, V.V. Pogozheva, M.A. Sukonnikov, E.A. Rogozhin, I.A. Prokhorenko, V.A. Brylev, A.M. Korolev, M.S. Slyundina, R.S. Borisov, M.V. Serebryakova, M.V. Shuvalov, A.L. Ksenofontov, L.G. Stoyanova, I.A. Osterman, A.A. Formanovsky, V.N. Tashlitsky, L.A. Baratova, A.V. Timofeeva, A.P. Tyurin, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 6, pp. 732–740.

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Lapchinskaya, O.A., Katrukha, G.S., Gladkikh, E.G. et al. Investigation of the complex antibiotic INA-5812. Russ J Bioorg Chem 42, 664–671 (2016).

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  • microbial metabolites
  • glycopeptide antibiotics
  • high performance liquid chromatography
  • high-resolution mass spectrometry