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Investigation of the complex antibiotic INA-5812

Russian Journal of Bioorganic Chemistry volume 42, pages 664–671 (2016)Cite this article

Abstract

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study.

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Abbreviations

CFU:

colony-forming unit

rpHPLC:

reversedphase high performance liquid chromatography

DEAE:

diethylaminoethyl

HRESI-MS:

high-resolution electrospray ionization mass spectrometry

MALDI-TOF:

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

TFA:

trifluoroacetic acid

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Authors and Affiliations

  1. Gause Institute of New Antibiotics, Moscow, 119021, Russia

    O. A. Lapchinskaya, G. S. Katrukha, E. G. Gladkikh, V. V. Kulyaeva, V. A. Alferova, V. V. Pogozheva, E. A. Rogozhin, I. A. Prokhorenko, A. M. Korolev, M. S. Slyundina, M. V. Shuvalov & A. P. Tyurin

  2. Federal Research Center of Nutrition and Biotechnology, Moscow, 109240, Russia

    P. V. Coodan

  3. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia

    A. P. Topolyan, E. A. Rogozhin, I. A. Prokhorenko, V. A. Brylev, A. A. Formanovsky & A. P. Tyurin

  4. Faculty of Chemistry, Moscow State University, Moscow, 119991, Russia

    M. A. Sukonnikov, M. V. Shuvalov, I. A. Osterman & V. N. Tashlitsky

  5. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, 119991, Russia

    M. S. Slyundina & R. S. Borisov

  6. Faculty of Physics, Mathematics and Natural Sciences, Russian University of Peoples’ Friendship, Moscow, 115419, Russia

    R. S. Borisov

  7. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia

    M. V. Serebryakova, A. L. Ksenofontov, I. A. Osterman, L. A. Baratova & A. V. Timofeeva

  8. Faculty of Biology, Moscow State University, Moscow, 119991, Russia

    L. G. Stoyanova

Authors
  1. O. A. Lapchinskaya
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  2. G. S. Katrukha
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  3. E. G. Gladkikh
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  4. V. V. Kulyaeva
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  5. P. V. Coodan
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  6. A. P. Topolyan
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  7. V. A. Alferova
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  8. V. V. Pogozheva
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  9. M. A. Sukonnikov
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  10. E. A. Rogozhin
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  11. I. A. Prokhorenko
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  12. V. A. Brylev
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  13. A. M. Korolev
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  14. M. S. Slyundina
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  15. R. S. Borisov
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  16. M. V. Serebryakova
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  17. M. V. Shuvalov
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  18. A. L. Ksenofontov
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  19. L. G. Stoyanova
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  20. I. A. Osterman
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  21. A. A. Formanovsky
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  22. V. N. Tashlitsky
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  23. L. A. Baratova
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  24. A. V. Timofeeva
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  25. A. P. Tyurin
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Corresponding author

Correspondence to A. P. Tyurin.

Additional information

Original Russian Text © O.A. Lapchinskaya, G.S. Katrukha, E.G. Gladkikh, V.V. Kulyaeva, P.V. Coodan, A.P. Topolyan, V.A. Alferova, V.V. Pogozheva, M.A. Sukonnikov, E.A. Rogozhin, I.A. Prokhorenko, V.A. Brylev, A.M. Korolev, M.S. Slyundina, R.S. Borisov, M.V. Serebryakova, M.V. Shuvalov, A.L. Ksenofontov, L.G. Stoyanova, I.A. Osterman, A.A. Formanovsky, V.N. Tashlitsky, L.A. Baratova, A.V. Timofeeva, A.P. Tyurin, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 6, pp. 732–740.

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Lapchinskaya, O.A., Katrukha, G.S., Gladkikh, E.G. et al. Investigation of the complex antibiotic INA-5812. Russ J Bioorg Chem 42, 664–671 (2016). https://doi.org/10.1134/S1068162016060078

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  • DOI: https://doi.org/10.1134/S1068162016060078

Keywords

  • microbial metabolites
  • glycopeptide antibiotics
  • high performance liquid chromatography
  • high-resolution mass spectrometry