Abstract
Infectious diseases caused by bacterial or viral agents represent the major cause of human pathogenesis and mortality worldwide. A development of novel antibacterial therapeutics and diagnostic tools is a very acute task. The use of DNA and RNA aptamers targeted to certain bacteria could be a promising solution to this problem. Here, we propose a new protocol of selection of 2′-fluoro RNA aptamers capable to internalize into bacterial cells. Using whole-cell SELEX against Pseudomonas aeruginosa, enriched 2′-fluoro RNA library was obtained, and its sequencing and data analysis were fulfilled. It was found that the central region of predominating aptamer sequence is identical to the fragment of P. aeruginosa rRNA. A possibility of internalizing of this aptamer into bacterial cells is shown. It is hypothesized that aptamers could be internalized more effectively as heterodimeric complexes.
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Abbreviations
- BSA:
-
bovine serum albumin
- CFU:
-
colony forming unit
- rRNA:
-
ribosomal RNA
- RT-PCR:
-
reverse transcription—polymerase chain reaction
- SELEX:
-
Systematic Evolution of Ligands by exponential enrichment
- tRNA:
-
transport RNA
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Original Russian Text © A.S. Davydova, M.A. Vorobyeva, M.R. Kabilov, N.V. Tikunova, D.V. Pyshnyi, A.G. Venyaminova, 2017, published in Bioorganicheskaya Khimiya, 2017, Vol. 43, No. 1, pp. 68–74.
The paper is based on the materials of the “Chemical Biology 2016” conference; Novosibirsk, Russia, July 24–29, 2016.
The article was translated by the authors.
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Davydova, A.S., Vorobyeva, M.A., Kabilov, M.R. et al. In vitro selection of cell-internalizing 2′-modified RNA aptamers against Pseudomonas aeruginosa . Russ J Bioorg Chem 43, 58–63 (2017). https://doi.org/10.1134/S1068162016060030
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DOI: https://doi.org/10.1134/S1068162016060030