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Development, optimization, and biovalidation of 99mTc–insulin complex


Human biosynthetic insulin is a polypeptide hormone that plays an important and essential role in control of the level of carbohydrate, protein, and fat metabolism in the blood. Human pancreatic insulin was labeled with 99mTc to form a new radiopharmaceutical with a labeling yield of 99 ± 1% under optimum conditions: 0.1 mL insulin, pH 7, 25 μg stannous chloride, 1 mL (19 mCi) of pertechnetate, room temperature, and 10 min reaction time. The 99mTc–insulin complex was examined using paper chromatography, ITLC, electrophoresis, and HPLC. In addition, in vitro and in vivo study of 99mTc–insulin complex was performed at different time intervals.

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Correspondence to Muhammad Usman Akbar.

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Akbar, M.U., Bokhari, T.H., Roohi, S. et al. Development, optimization, and biovalidation of 99mTc–insulin complex. Russ J Bioorg Chem 42, 491–496 (2016).

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  • insulin
  • technetium-99m
  • labeling
  • optimization
  • HPLC
  • electrophoresis
  • biodistribution