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Development, optimization, and biovalidation of 99mTc–insulin complex

Russian Journal of Bioorganic Chemistry volume 42, pages 491–496 (2016)Cite this article

Abstract

Human biosynthetic insulin is a polypeptide hormone that plays an important and essential role in control of the level of carbohydrate, protein, and fat metabolism in the blood. Human pancreatic insulin was labeled with 99mTc to form a new radiopharmaceutical with a labeling yield of 99 ± 1% under optimum conditions: 0.1 mL insulin, pH 7, 25 μg stannous chloride, 1 mL (19 mCi) of pertechnetate, room temperature, and 10 min reaction time. The 99mTc–insulin complex was examined using paper chromatography, ITLC, electrophoresis, and HPLC. In addition, in vitro and in vivo study of 99mTc–insulin complex was performed at different time intervals.

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Authors and Affiliations

  1. Department of Chemistry, Government College University, Faisalabad, 38000, Pakistan

    Muhammad Usman Akbar, Tanveer Hussain Bokhari & Narmeen Ali

  2. Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, 38000, Pakistan

    Muhammad Usman Akbar, Khalid Mahmood Zia & Mohammad Zuber

  3. Isotope Production Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad, Pakistan

    Samina Roohi

  4. Pakistan School, Muscat, Sultanate of Oman

    Nadia Parveen

Authors
  1. Muhammad Usman Akbar
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  2. Tanveer Hussain Bokhari
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  3. Samina Roohi
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  4. Khalid Mahmood Zia
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  5. Mohammad Zuber
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  6. Nadia Parveen
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  7. Narmeen Ali
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Correspondence to Muhammad Usman Akbar.

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Akbar, M.U., Bokhari, T.H., Roohi, S. et al. Development, optimization, and biovalidation of 99mTc–insulin complex. Russ J Bioorg Chem 42, 491–496 (2016). https://doi.org/10.1134/S1068162016050125

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  • DOI: https://doi.org/10.1134/S1068162016050125

Keywords

  • insulin
  • technetium-99m
  • labeling
  • optimization
  • HPLC
  • electrophoresis
  • biodistribution