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Effect of lipoic acid on the activity of caspases and the characteristics of the immune and antioxidant statuses in rats with rheumatoid arthritis

Abstract

The activity of caspase-3, caspase-8, superoxide dismutase, and catalase and the content of circulating immune complexes and immunoglobulins A, M, and G in the blood serum and muscles of rats in the norm, in experimental rheumatoid arthritis, and by the action of lipoic acid against the background of progressive pathology have been studied. The induction of rheumatoid arthritis in rats was accompanied by an increase in marker parameters and immune status indices, as well as in the activity of caspase-3, caspase-8, superoxide dismutase, and catalase, which was probably caused by excessive generation of reactive oxygen species during the development of pathology. The administration of lipoic acid to rats with rheumatoid arthritis led to a normalization of the parameters, which was evidently due to the capacity of the compound to produce the antioxidant effect and reduce the severity of autoimmune diseases.

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Abbreviations

CIC:

circulating immune complexes

DHLA:

dihydrolipoic acid

FRO:

free-radical oxidation

LA:

lipoic acid

LPO:

lipid peroxidation

NBT:

nitro blue tetrazolium

PMS:

phenazine methosulfate

RA:

rheumatoid arthritis

RF:

rheumatoid factor

ROS:

reactive oxygen species

SOD:

superoxide dismutase

ESR:

erythrocyte sedimentation rate

References

  1. Mazurov, V.I., Klinicheskaya revmatologiya: rukovodstvo dlya vrachei (Clinical Rheumatology: A Guide for Physicians), St. Petersburg: Foliant, 2005.

    Google Scholar 

  2. Hirao, M., Yamasaki, N., Oze, H., Ebina, K., Nampei, A., Kawato, Y., Shi, K., Yoshikawa, H., Nishimoto, N., and Hashimoto, J., Rheumatol. Int., 2012, vol. 32, pp. 4041–4045.

    CAS  Article  PubMed  Google Scholar 

  3. Rho, Y.H., Chung, C.P., Oeser, A., Solus, J.F., Gebretsadik, T., Shintani, A., Raggi, P., Milne, G.L., and Stein, C.M., Arthritis Care Res., 2010, vol. 62, no. 10, pp. 1473–1480.

    CAS  Article  Google Scholar 

  4. Popova, T.N., Agarkov, A.A., and Verevkin, A.N., Acta Naturae, 2013, vol. 5, no. 4 (19), pp. 129–134.

    Google Scholar 

  5. Tchetina, E.V., Demidova, N.V., Karateev, D.E., and Nasonov, E.L., Int. J. Rheumatol., 2013, vol. 2013, Article ID457876.

  6. Martinova, E.A., Russ. J. Bioorg. Chem., 2003, vol. 29, no. 5, pp. 471–495.

    CAS  Article  Google Scholar 

  7. Tien, Y.C., Liao, J.C., Chiu, C.S., Huang, T.H., Huang, C.Y., Chang, W.T., and Peng, W.H., Int. J. Mol. Sci., 2011, vol. 1, no. 2, pp. 4053–4067.

    Article  Google Scholar 

  8. Bayati, S. and Yazdanparast, R., Iranian Biomed. J., 2011, vol. 15, no. 4, pp. 134–142.

    CAS  Google Scholar 

  9. Liang, H.L., Arsenault, J., Mortensen, J., Park, F., Johnson, C.P., and Nilakantan, V., Apoptosis, 2009, vol. 14, no.10.

    Google Scholar 

  10. Swaran, J.S., Flora, Oxid. Med. Cell. Longev., 2009, vol. 2, no. 4, pp. 191–206.

    Article  Google Scholar 

  11. Liang, J.F., Chem. Biol. Interact., 2000, vol. 124, no. 1, pp. 53–60.

    CAS  Article  PubMed  Google Scholar 

  12. Zacharias, E., Pharm. Res., 2003, no. 48, pp. 585–591.

    Article  Google Scholar 

  13. Gomes, M.B. and Negrato, C.A., Diabetol. Metab. Syndr., 2014, vol. 6, no.80.

    Google Scholar 

  14. Mesquitaa, A., Weinbergerb, M., Silvaa, A., Sampaio-Marquesa, B., Almeidaa, B., Leaoa, C., Costa, V., Rodriguesa, F., Burhans, W.C., and Ludovico, P., Proc. Natl. Acad. Sci. U. S. A., 2010, vol. 107, no. 34, pp. 15123–15128.

    Article  Google Scholar 

  15. Holers, V.M., Curr. Opin. Immunol., 2013, vol. 25, no. 6, pp. 728–735.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. Van Steendam, K., Tilleman, K., De Ceuleneer, M., De Keyser, F., Elewaut, D., and Deforce, D., Arthritis Res. Ther., 2010, vol. 12, no. R132.

    Google Scholar 

  17. Salintho, S., Yadav, V., Schillace, R.V., Bourdette, D.N., and Carr, D.W., PLoS ONE, 2010, vol. 5, no.9.

    Google Scholar 

  18. Yadav, V., Marracci, G.H., Munar, M.Y., Cherala, G., Stuber, L.E., Alvarez, L., Shinto, L., Koop, D.R., and Bourdette, D.N., Mult. Scler., 2010, vol. 16, no. 4, pp. 387–397.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. Deneke, S.M., Curr. Top. Cell Regul., 2000, vol. 36, pp. 151–180.

    CAS  Article  PubMed  Google Scholar 

  20. Self, W.T., Tsai, L., and Stadtman, T.C., Proc. Natl. Acad. Sci. U. S. A., 2000, vol. 97, pp. 12481–12486.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. Pierce, R.H., Campbell, J.S., Stephenson, A.B., Franklin, C.C., Chaisson, M., Poot, M., Kavanagh, T.J., Rabinovitch, P.S., and Fausto, N., Am. J. Pathol., 2000, vol. 157, no. 1, pp. 221–236.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. Kaya-Dagistanli, F., Tanriverdi, G., Altinok, A., Ozyazgan, S., and Ozturk, M., Food Chem. Toxicol., 2013, vol. 53, pp. 84–93.

    CAS  Article  PubMed  Google Scholar 

  23. Bobyrev, V.N., Pochernyaeea, V.F., Starodubtsev, S.G., Bobyreva, L.E., Dubinskaya, G.M., and Voskresenskii, O.N., Eksp. Klin. Farmakol., 1994, vol. 57, no. 1, pp. 47–54.

    CAS  PubMed  Google Scholar 

  24. Filippin, L.I., Vercelino, R., Marroni, N.P., and Xavier, R.M., Clin. Exp. Immunol., 2008, vol. 152, pp. 415–422.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. Frei, B., Stocker, R., and Ames, B., Proc. Natl. Acad. Sci. U. S. A., 1988, vol. 85, pp. 9748–9752.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  26. Feng, B., Yan, X.F., Xue, J.L., Xu, L., and Wang, H., Int. J. Mol. Sci., 2013, vol. 14, no. 4, pp. 6746–6756.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  27. Vershinin, V.I., Vlasova, I.V., and Nikiforova, I.A., Analiticheskaya khimiya (Analytical Chemistry), Moscow: Akademiya, 2011.

    Google Scholar 

  28. Lugovskaya, S.A., Morozova, V.T., Pochtar’, M.E., and Dolgov, V.V., Laboratornaya gematologiya (Laboratory Hematology), Tver: Triada, 2014.

    Google Scholar 

  29. White, A., Handler, P., Smith, E., Hill, R., and Lehman, I.R., Principles of Biochemistry, New York: McGrow-Hill, 1978.

    Google Scholar 

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Correspondence to E. D. Kryl’skiy.

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Original Russian Text © E.D. Kryl’skiy, T.N. Popova, E.M. Kirilova, O.A. Safonova, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 4, pp. 431–439.

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Kryl’skiy, E.D., Popova, T.N., Kirilova, E.M. et al. Effect of lipoic acid on the activity of caspases and the characteristics of the immune and antioxidant statuses in rats with rheumatoid arthritis. Russ J Bioorg Chem 42, 389–396 (2016). https://doi.org/10.1134/S1068162016040130

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  • DOI: https://doi.org/10.1134/S1068162016040130

Keywords

  • rheumatoid arthritis
  • free radical oxidation
  • caspases
  • antioxidant system
  • lipoic acid