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The use of H/D exchange for secondary structure characterization of supermetallized complexes of ubiquitin with cerium(III)

Russian Journal of Bioorganic Chemistry volume 42, pages 484–490 (2016)Cite this article

Abstract

The approach of hydrogen/deuterium exchange combined with ultrahigh resolution mass spectrometry was applied for investigation of conformational changes of supermetallized ubiquitin ions with cerium(III) atoms. The dependencies of the hydrogen/deuterium exchange efficiency on the charge state of ubiquitin ion, the number of associated cerium atoms, as well as on the temperature were obtained. The reaction of hydrogen/deuterium exchange was performed directly in the ionization source according to previously described method. It was found that the number of exchanges is hardly altered under the addition of cerium atoms. This result indirectly suggests that the conformation of small protein supermetallized ions does not significantly change during electrospray ionization.

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Abbreviations

ICR:

ion cyclotron resonance

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Authors and Affiliations

  1. Skolkovo Institute of Science and Technology, ul. Novaya 100, Skolkovo, Moscow oblast, 143025, Russia

    Yu. Kostyukevich & E. Nikolaev

  2. Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskii pr. 38, k. 2, Moscow, 119334, Russia

    Yu. Kostyukevich, A. Kononikhin & E. Nikolaev

  3. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia

    Yu. Kostyukevich, I. Popov & A. Bugrova

  4. Moscow Institute of Physics and Technology, State University, Institutskii per. 9, Dolgoprudny, Moscow oblast, 141700, Russia

    P. Yacovlev, A. Kononikhin, I. Popov, N. Starodubtzeva & E. Nikolaev

  5. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, ul. Oparina 4, Moscow, 117997, Russia

    A. Kononikhin, A. Bugrova & N. Starodubtzeva

Authors
  1. Yu. Kostyukevich
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  2. P. Yacovlev
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  3. A. Kononikhin
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  4. I. Popov
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  5. A. Bugrova
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  6. N. Starodubtzeva
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  7. E. Nikolaev
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Corresponding author

Correspondence to E. Nikolaev.

Additional information

Original Russian Text © Yu. Kostyukevich, P. Yacovlev, A. Kononikhin, I. Popov, A. Bugrova, N. Starodubtzeva, E. Nikolaev, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 5, pp. 539–545.

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Kostyukevich, Y., Yacovlev, P., Kononikhin, A. et al. The use of H/D exchange for secondary structure characterization of supermetallized complexes of ubiquitin with cerium(III). Russ J Bioorg Chem 42, 484–490 (2016). https://doi.org/10.1134/S1068162016040117

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  • DOI: https://doi.org/10.1134/S1068162016040117

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