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Radioligand method of assessment of human T-lymphocytes’ β-adrenoceptors activity

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Abstract

A new method of evaluation of beta-receptor’s activity on the surface of human T-lymphocytes has been proposed based on the radioligand method. Optimal conditions for evaluation of specific binding to β2-adrenoceptors of 0.5 fmol ligand per 1 million cells using [125I] -cyanopindolol were found. The possibility of using β2-adrenoceptor’s activity assessment in clinical settings was demonstrated on human T-lymphocytes.

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Abbreviations

PBS:

5 mM potassium phosphate buffer with 0.14 M NaCl

pH 7.0; BSA:

bovine serum albumin

PKA:

protein kinase A

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Authors and Affiliations

  1. Laboratory of Pulmonology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, ul. Delegatskaya 20/1, Moscow, 127473, Russia

    O. Y. Agapova, K. A. Zykov, A. V. Rvacheva & V. B. Beilina

  2. Laboratory of Isotope Analysis, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS (IBCh RAS), ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    Y. S. Skoblov

  3. Russian Cardiology Research and Production Complex, ul. Tret’ya Cherepkovskaya 15A, Moscow, 121552, Russia

    V. P. Masenko & I. E. Chazova

Authors
  1. O. Y. Agapova
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  2. Y. S. Skoblov
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  3. K. A. Zykov
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  4. A. V. Rvacheva
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  5. V. B. Beilina
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  6. V. P. Masenko
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  7. I. E. Chazova
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Correspondence to Y. S. Skoblov.

Additional information

Original Russian Text © O.Y. Agapova, Y.S. Skoblov, K.A. Zykov, A.V. Rvacheva, V.B. Beilina, V.P. Masenko, I.E. Chazova, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 5, pp. 592–598.

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Agapova, O.Y., Skoblov, Y.S., Zykov, K.A. et al. Radioligand method of assessment of human T-lymphocytes’ β-adrenoceptors activity. Russ J Bioorg Chem 41, 529–535 (2015). https://doi.org/10.1134/S1068162015050027

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  • DOI: https://doi.org/10.1134/S1068162015050027

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