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On-microchip PCR for detection of subtypes of influenza a viruses circulating in the human population

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Abstract

An oligonucleotide microchip was developed for the detection of the Influenza A virus subtypes circulating in the human population, i.e., pandemic H1N1 swine influenza virus and seasonal H1N1, H2N2, H3N2, H5N1, H9N2, and H7N9 viruses. The typing of influenza viruses was performed using an on-microchip PCR. We used immobilized primer probes chosen for the neuraminidase gene, which allowed determining both neuraminidase and hemagglutinin subtypes.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrent’eva 8, Novosibirsk, 630090, Russia

    E. V. Kostina, V. A. Ryabinin & A. N. Sinyakov

  2. Department of Molecular Virology, FBRI State Research Center of Virology and Biotechnology “Vector,” Koltsovo, Novosibirsk oblast, 630559, Russia

    V. A. Ternovoi

Authors
  1. E. V. Kostina
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  2. V. A. Ryabinin
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  3. V. A. Ternovoi
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  4. A. N. Sinyakov
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Corresponding author

Correspondence to E. V. Kostina.

Additional information

Original Russian Text © E.V. Kostina, V.A. Ryabinin, V.A. Ternovoi, A.N. Sinyakov, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 1, pp. 124–128.

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Kostina, E.V., Ryabinin, V.A., Ternovoi, V.A. et al. On-microchip PCR for detection of subtypes of influenza a viruses circulating in the human population. Russ J Bioorg Chem 41, 108–111 (2015). https://doi.org/10.1134/S1068162015010082

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  • DOI: https://doi.org/10.1134/S1068162015010082

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