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Expression of house dust mite allergens Der f 1 and Der f 2 in leaves of Nicotiana benthamiana

Russian Journal of Bioorganic Chemistry volume 40, pages 433–442 (2014)Cite this article

Abstract

In test systems for diagnostics of type I allergy, recombinant allergens in native conformation are used, because immunoglobulins E (IgE), responsible for the development of this type of allergy, recognize conformational epitopes of protein allergens. To obtain recombinant allergens of the Dermatophagoides farinae house dust mites (HDM), Der f 1 and Der f 2, two systems of heterological expression were used: Escherichia coli and Nicotiana benthamiana plants. IgE from sera of children allergic to HDM were shown to recognize the recombinant Der f 2 protein synthesized in both E. coli and N. benthamiana plants, as well as the recombinant Der f 1 protein produced in N. benthamiana plants, while mature form of Der f 1 produced in E. coli did not interact with IgE.

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Abbreviations

HDM:

house dust mites

SIT:

specific immunotherapy

IgE:

immunoglobulin class E

References

  1. Platt-Mills, T.A.E. and Chapman, M.D., J. Allergy Clin. Immunol., 1987, vol. 80, pp. 755–775.

    Article  Google Scholar 

  2. Thomas, W.R., Smith, W.A., Hales, B.J., Mills, K.L., and O’Brien, R.M., Int. Arch. Allergy Immunol., 2002, vol. 129, pp. 1–18.

    CAS  PubMed  Article  Google Scholar 

  3. Kawamoto, S., Aki, T., Yamashita, M., Tategaki, A., Fujimura, T., Tsuboi, S., Katsutani, T., Suzuki, O., Shigeta, S., Murooka, Y., et al., J. Biosci. Bioeng., 2002, vol. 94, pp. 285–298.

    CAS  PubMed  Article  Google Scholar 

  4. Takai, T., Kato, T., Ota, M., Yasueda, H., Kuhara, T., Okumura, K., and Ogawa, H., Int. Arch. Allergy Immunol., 2005, vol. 137, pp. 194–200.

    CAS  PubMed  Article  Google Scholar 

  5. Meno, K., Thorsted, P.B., Ipsen, H., Kristensen, O., Larsen, J.N., Spangfort, M.D., Gajhede, M., and Lund, K., J. Immunol., 2005, vol. 175, pp. 3835–3845.

    CAS  PubMed  Article  Google Scholar 

  6. Takai, T., Mizuuchi, E., Kituchi, Y., Nagamune, T., Okumura, K., and Ogawa, H., Int. Arch. Allergy Immunol., 2006, vol. 139, pp. 181–187.

    CAS  PubMed  Article  Google Scholar 

  7. van Oort, E., de Heer, P.G., van Leeuwen, W.A., Derksen, N.I., Muller, M., Huveneers, S., Aalberse, R.C., and van Ree, R., Eur. J. Biochem., 2002, vol. 269, pp. 671–679.

    PubMed  Article  Google Scholar 

  8. Chevigne, A., Barumandzadeh, R., Groslambert, S., Cloes, B., Dehareng, D., Filee, P., Marx, J.C., Frere, J.M., Matagne, A., Jacquet, A., and Galleni, M., J. Mol. Biol., 2007, vol. 374, pp. 170–185.

    CAS  PubMed  Article  Google Scholar 

  9. Pichavant, M., Charbonnier, A.S., Taront, S., Brichet, A., Wallaert, B., Pestel, J., Tonnel, A.B., and Gosset, P., J. Allergy Clin. Immunol., 2005, vol. 115, pp. 771–778.

    CAS  PubMed  Article  Google Scholar 

  10. Lombardero, M., Heymann, P.W., Platts-Mills, T.A., Fox, J.W., and Chapman, M.D., J. Immunol., 1990, vol. 144, pp. 1353–1360.

    CAS  PubMed  Google Scholar 

  11. Hales, B.J., Martin, A.C., Pearce, L.J., Laing, I.A., Hayden, C.M., Goldblatt, J., Le Souef, P.N., and Thomas, W., J. Allergy Clin. Immunol., 2006, vol. 118, pp. 361–367.

    CAS  PubMed  Article  Google Scholar 

  12. Yuuki, T., Okumura, Y., Ando, T., Yamakawa, H., Suko, M., Haida, M., Dohi, M., and Okudaira, H., Agric. Biol. Chem., 1991, vol. 55, pp. 1233–1238.

    CAS  PubMed  Article  Google Scholar 

  13. Thomas, W.R. and Chua, K.Y., Clin. Exp. Allergy, 1995, vol. 25, pp. 667–669.

    CAS  PubMed  Article  Google Scholar 

  14. Chua, K.Y., Greene, W.K., Kehal, P., and Thomas, W.R., Clin. Exp. Allergy, 1991, vol. 21, pp. 161–166.

    CAS  PubMed  Article  Google Scholar 

  15. Hof, W., Driedijk, P.C., van de Berg, M., Beck-Sickinger, A.G., Jung, G., and Aalberse, R.C., Mol. Immunol., 1991, vol. 28, pp. 1225–1232.

    Article  Google Scholar 

  16. Nishiyama, C., Fukuda, M., Usui, Y., Iwamoto, N., Yuuki, T., Okumura, Y., and Okudaira, H., Mol. Immunol., 1995, vol. 32, pp. 1021–1029.

    CAS  PubMed  Article  Google Scholar 

  17. Chua, K.Y., Dilworth, R.J., and Thomas, W.R., Int. Arch. Allergy Appl. Immunol., 1990, vol. 91, pp. 124–129.

    CAS  PubMed  Article  Google Scholar 

  18. Hoyne, G., Bourne, T., Kristensen, N., Hetzel, C., and Lamb, J., Clin. Immunol. Immunopathol., 1996, vol. 80, pp. S23–S30.

    CAS  PubMed  Article  Google Scholar 

  19. Batard, T., Didierlaurent, A., Chabre, H., Mothes, N., Bussieres, L., Bohle, B., Couret, M.N., Ball, T., Lemoine, P., Focks, TejklM., et al., Int. Arch. Allergy Immunol., 2005, vol. 136, pp. 239–249.

    CAS  PubMed  Article  Google Scholar 

  20. Valenta, R., Ferreira, F., Focke-Tejkl, M., Linhart, B., Niederberger, V., Swoboda, I., and Vrtala, S., Ann. Rev. Immunol., 2010, vol. 28, pp. 211–241.

    CAS  Article  Google Scholar 

  21. Ferreira, F., Wallner, M., Breiteneder, H., Hartl, A., Thalhamer, J., and Ebner, C., Int. Arch. Allergy Immunol., 2002, vol. 128, pp. 171–178.

    CAS  PubMed  Article  Google Scholar 

  22. Mueller, G.A., Smith, A.M., Williams, D.C., Jr., Hakkaart, G.A., Aalberse, R.C., Chapman, M.D., Rule, G.S., and Benjamin, D.C., J. Biol. Chem., 1997, vol. 272, pp. 26893–26898.

    CAS  PubMed  Article  Google Scholar 

  23. Hakkaart, G.A., Harmsen, M.M., Chua, K.Y., Thomas, W.R., Aalberse, R.C., and Van Ree, R., Clin. Exp. Allergy, 1998, vol. 28, pp. 45–52.

    CAS  PubMed  Article  Google Scholar 

  24. Jacquet, A., Haumont, M., Massaer, M., Daminet, V., Garcia, L., Mazzu, P., Jacobs, P., and Bollen, A., Clin. Exp. Allergy, 2000, vol. 30, pp. 677–684.

    CAS  PubMed  Article  Google Scholar 

  25. Jacquet, A., Magi, M., Petry, H., and Bollen, A., Clin. Exp. Allergy, 2002, vol. 32, pp. 1048–1053.

    CAS  PubMed  Article  Google Scholar 

  26. Massaer, M., Mazzu, P., Haumont, M., Magi, M., Daminet, V., Bollen, A., and Jacquet, A., Int. Arch. Allergy Immunol., 2001, vol. 125, pp. 32–43.

    CAS  PubMed  Article  Google Scholar 

  27. Cui, Y., Zhou, P., Peng, J., Peng, M., Zhou, Y., Lin, Y., and Liu, L., Braz. J. Med. Biol. Res., 2008, vol. 41, pp. 380–388.

    CAS  PubMed  Article  Google Scholar 

  28. de Halleux, S., Stura, E., Vanderelst, L., Carlier, V., Jacquemin, M., and Saint-Remy, J.M., J. Allergy Clin. Immunol., 2006, vol. 117, pp. 571–576.

    PubMed  Article  Google Scholar 

  29. Yasuhara, T., Takai, T., Yuuki, T., Okudaira, H., and Okumura, Y., Clin. Exp. Allergy, 2001, vol. 31, pp. 116–124.

    CAS  PubMed  Article  Google Scholar 

  30. Best, E.A., Stedman, K.E., Bozic, C.M., Hunter, S.W., Vailes, L., Chapman, M.D., McCall, C.A., and McDermott, M.J., Protein Expr. Purif., 2000, vol. 20, pp. 462–471.

    CAS  PubMed  Article  Google Scholar 

  31. Burtin, D., Chabre, H., Olagnier, B., Didierlaurent, A., Couret, M.N., Comeau, D., Wambre, E., Laparra, H., Van Overtvelt, L., Montandon, F., et al., Clin. Exp. Allergy, 2009, vol. 39, pp. 760–770.

    CAS  PubMed  Article  Google Scholar 

  32. Komarova, T.V., Citovsky, V., and Dorokhov, Y.L., RNAi Technology, Gaur, R.K., Gafni, Y., Sharma, P., and Gupta, V.K., Eds., CRC Press, Taylor and Francis Group, USA, 2011, pp. 125–134.

  33. Takahashi, K., Takai, T., Yasuhara, T., Yuuki, T., Ohtake, Y., Yokota, T., and Okumura, Y., Int. Arch. Allergy Immunol., 2000, vol. 122, pp. 108–114.

    CAS  PubMed  Article  Google Scholar 

  34. Yang, L., Kajiura, H., Suzuki, K., Hirose, S., Fujiyama, K., and Takaiwa, F., Biochem. Biophys. Res. Commun., 2008, vol. 365, pp. 334–339.

    CAS  PubMed  Article  Google Scholar 

  35. Yang, L., Hirose, S., Suzuki, K., Hiroi, T., and Takaiwa, F., J. Exp. Bot., 2012, vol. 63, pp. 2947–2959.

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  36. Kurup, V.P., Knutsen, A.P., Moss, R.B., and Bansal, N.K., Clin. Mol. Allergy, 2006, vol. 4, p. 11.

    PubMed Central  PubMed  Article  Google Scholar 

  37. Sambrook, J. and Russell, D.W., CSHL Press, 2001.

    Google Scholar 

  38. Laemmli, U.K., Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    CAS  PubMed  Article  Google Scholar 

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    D. Yu. Ryazantsev, P. E. Drobyazina, S. K. Zavriev & E. V. Svirshchevkaya

  2. All-Russian Research Institute for Agricultural Biotechnology, Russian Academy of Agricultural Sciences, ul. Timiryazevskaya 42, Moscow, 127550, Russia

    P. E. Drobyazina

  3. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Malyi Kazennyi per. 5a, Moscow, 105064, Russia

    S. V. Khlgatyan

Authors
  1. D. Yu. Ryazantsev
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  2. P. E. Drobyazina
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  3. S. V. Khlgatyan
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  4. S. K. Zavriev
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  5. E. V. Svirshchevkaya
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Correspondence to D. Yu. Ryazantsev.

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Original Russian Text © D.Yu. Ryazantsev, P.E. Drobyazina, S.V. Khlgatyan, S.K. Zavriev, E.V. Svirshchevkaya, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 4, pp. 468–478.

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Ryazantsev, D.Y., Drobyazina, P.E., Khlgatyan, S.V. et al. Expression of house dust mite allergens Der f 1 and Der f 2 in leaves of Nicotiana benthamiana . Russ J Bioorg Chem 40, 433–442 (2014). https://doi.org/10.1134/S1068162014040116

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  • DOI: https://doi.org/10.1134/S1068162014040116

Keywords

  • allergy
  • house dust mites
  • Der f 1
  • Der f 2
  • agroinfiltration
  • immunoglobulin E