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Expression of house dust mite allergens Der f 1 and Der f 2 in leaves of Nicotiana benthamiana

Abstract

In test systems for diagnostics of type I allergy, recombinant allergens in native conformation are used, because immunoglobulins E (IgE), responsible for the development of this type of allergy, recognize conformational epitopes of protein allergens. To obtain recombinant allergens of the Dermatophagoides farinae house dust mites (HDM), Der f 1 and Der f 2, two systems of heterological expression were used: Escherichia coli and Nicotiana benthamiana plants. IgE from sera of children allergic to HDM were shown to recognize the recombinant Der f 2 protein synthesized in both E. coli and N. benthamiana plants, as well as the recombinant Der f 1 protein produced in N. benthamiana plants, while mature form of Der f 1 produced in E. coli did not interact with IgE.

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Abbreviations

HDM:

house dust mites

SIT:

specific immunotherapy

IgE:

immunoglobulin class E

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Correspondence to D. Yu. Ryazantsev.

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Original Russian Text © D.Yu. Ryazantsev, P.E. Drobyazina, S.V. Khlgatyan, S.K. Zavriev, E.V. Svirshchevkaya, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 4, pp. 468–478.

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Ryazantsev, D.Y., Drobyazina, P.E., Khlgatyan, S.V. et al. Expression of house dust mite allergens Der f 1 and Der f 2 in leaves of Nicotiana benthamiana . Russ J Bioorg Chem 40, 433–442 (2014). https://doi.org/10.1134/S1068162014040116

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  • DOI: https://doi.org/10.1134/S1068162014040116

Keywords

  • allergy
  • house dust mites
  • Der f 1
  • Der f 2
  • agroinfiltration
  • immunoglobulin E