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Mulberry (Morus L.) methionine sulfoxide reductase gene cloning, sequence analysis, and expression in plant development and stress response

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Abstract

Methionine sulfoxide reductase plays a regulatory role in plant growth and development, especially in scavenging reactive oxygen species by restoration of the oxidation of methionine in protein. A fulllength cDNA sequence encoding methionine sulfoxide reductase (MSR) from mulberry, which we designated MMSR, was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MMSR is 810 bp long, encoding 194 amino acids with a predicted molecular weight of 21.6 kDa and an isoelectric point of 6.78. The expression level of the MMSR gene under conditions of drought and salt stresses was quantified by qRT-PCR. The results show that the expression level changed significantly under the stress conditions compared to the normal growth environment. It helps us to get a better understanding of the molecular basis for signal transduction mechanisms underlying the stress response in mulberry.

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References

  1. Rouhier, N., Dos Santos, C.V., Tarrago, L., and Rey, P., Photosynth. Res., 2006, vol. 89, pp. 1463–1469.

    Article  Google Scholar 

  2. Cabreiro, F., Picot, C.R., Perichon, M., Castel, J., Friguet, B., and Petropoulos, I., J. Biol. Chem., 2008, vol. 283, pp. 1126–1134.

    Article  Google Scholar 

  3. Niklas, G., Bas, P., Kokke, U.H., Maris, S., Ulrike, B., Zaruhi, P., Denis, M., Wilbert, C., and Boelens, C.S., Plant J., 2002, vol. 29, pp. 2636–2643.

    Google Scholar 

  4. Stadtman, E.R., Moskovltz, J., Berlett, B.S., and Levine, R.L., Mol. Cell Biochem., 2002, vol. 234, pp. 3–9.

    Article  PubMed  Google Scholar 

  5. Wizemann, T.M., Moskovitz, J., Pearce, B.J., Cundell, D., Arvidson, C.G., So, M., Weissbach, H., Brot, N., and Masure, H.R., Proc. Natl. Acad. Sci. U.S.A., 1996, vol. 93, pp. 7985–7990.

    Article  PubMed  CAS  Google Scholar 

  6. Rahman, M., Nelson, H., Weissbach, H., and Brot, N., J. Biol. Chem., 1992, vol. 267, pp. 15549–15551.

    PubMed  CAS  Google Scholar 

  7. Moskovitz, J., Berlett, B.S., Poston, J.M., and Stadtman, R.R., Proc. Natl. Acad. Sci. U.S.A., 1997, vol. 94, pp. 9585–9589.

    Article  PubMed  CAS  Google Scholar 

  8. Sadanandom, A., Piffanelli, P., Knott, T., Robinson, C., Sharpe, A., Lydiate, D., Murphy, D., and Fairbairn, D.J., Plant J., 1996, vol. 10, pp. 235–242.

    Article  PubMed  CAS  Google Scholar 

  9. Sadanandom, A., Poghosyan, A., Fairbain, D.J., and Murphy, D.J., Plant Physiol., 2000, vol. 123, pp. 255–264.

    Article  PubMed  CAS  Google Scholar 

  10. Moskovitz, J., Weissbach, H., and Brot, N., Proc. Natl. Acad. Sci. U.S.A., 1996, vol. 93, pp. 2095–2099.

    Article  PubMed  CAS  Google Scholar 

  11. Petropoulos, I., Mary, J., Perchon, M., and Friguet, B., J. Biol. Chem., 2001, vol. 355, pp. 1262–1270.

    Google Scholar 

  12. Hu, P.R., Yu, L., Zhang, M., Zheng, L.H., Lan, F., Fu, Q., and Zhao, S.Y., Chin. Sci. Bull., 2000, vol. 45, pp. 369–376.

    Article  Google Scholar 

  13. Kuschel, L., Hansel, A., Schonhen, R., Weissbach, H., Brot, N., Hoshi, T., and Heinemann, S.H., FEBS Lett., 1999, vol. 456, pp. 17–21.

    Article  PubMed  CAS  Google Scholar 

  14. Jee, E., Hossain, M.d., Kim, J.H., Chu, S.H., Lee, E.H., Hwang, K.Y., Noh, H., Hong, S.W., and Lee, H., Plant Sci., 2010, vol. 178, pp. 1123–1132.

    Google Scholar 

  15. Zhao, J.Y., Zuo, K.J., Sun, X.F., and Tang, K.X., State Intellectual Property Office of China, 2004.

    Google Scholar 

  16. Lu, X.D., Song, S.F., Li, L.Y., Wang, M.L., and Xia, X.J., Res. Agricul. Modern., 2010, vol. 31, pp. 1143–1149.

    Google Scholar 

  17. Romero, H.M., Pell, E.J., and Tien, M., Plant, Sci., 2006, vol. 170, pp. 367–372.

    Article  Google Scholar 

  18. Fang, R.J., Qi, J.L., Hu, D.Q., and Zhao, W.G., Seri. Sci., 2008, vol. 34, pp. 581–586.

    CAS  Google Scholar 

  19. Zhao, W.G., Nanjing: Nanjing University Postdoctoral, 2008.

    Google Scholar 

  20. Gao, Y. and Zhao, Y., Plant Mol. Biol. Rep., 2010, vol. 28, pp. 450–459.

    Article  CAS  Google Scholar 

  21. Tamura, K., Dudley, J., Nei, M., and Kumar, S., Mol. Evol., 2007, vol. 24, pp. 1596–1599.

    Article  CAS  Google Scholar 

  22. Thompson, J.D., Higgins, D.G., and Gibson, T.J., Nucleic Acids Res., 2002, vol. 22, pp. 4673–4680.

    Article  Google Scholar 

  23. Zhao, D.Q. and Zhou, C.H., Plant Mol. Biol. Rep., 2010, vol. 29, pp. 345–351.

    Article  Google Scholar 

  24. Ranaivoson, F.M., Antoine, M., Kauffmann, B., Bosch-Muller, S., Aubry, A., Branlant, G., and Favier, F., J. Mol. Biol., 2008, vol. 377, pp. 12564–12571.

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Biological and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, 212018, P.R. China

    Wei Tong, Yinghua Zhang, Heng Wang, Feng Li, Zhaoyue Liu, Yuhua Wang, Rongjun Fang, Weiguo Zhao & Long Li

  2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, 212018, P.R. China

    Yuhua Wang, Weiguo Zhao & Long Li

  3. School of Life Science, Nanjing University, Nanjing, Jiangsu, 210093, P.R. China

    Rongjun Fang

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  1. Wei Tong
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  2. Yinghua Zhang
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  3. Heng Wang
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  4. Feng Li
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  5. Zhaoyue Liu
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  6. Yuhua Wang
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  7. Rongjun Fang
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  8. Weiguo Zhao
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  9. Long Li
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Correspondence to Weiguo Zhao or Long Li.

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The article is published in the original.

The first two authors contributed equally.

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Tong, W., Zhang, Y., Wang, H. et al. Mulberry (Morus L.) methionine sulfoxide reductase gene cloning, sequence analysis, and expression in plant development and stress response. Russ J Bioorg Chem 39, 522–529 (2013). https://doi.org/10.1134/S1068162013050154

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  • DOI: https://doi.org/10.1134/S1068162013050154

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