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Calpain system dysregulation in rat brain at beta-amyloid-induced neurodegeneration

Adapted from a report presented at the VI Russian Symposium “Proteins and Peptides” (June 11–15, 2013)

Abstract

Experimental evidence of calcium-dependent proteolysis dysregulation in brain of the murine model of Alzheimer’s disease was obtained. Experimental treatment, consisting of intra-hippocampal injection of amyloid beta-peptide (Aβ1–40), promoted activation of main calpain forms in murine brain along with a decrease in the content of a natural calpain inhibitor, calpastatin. As a result of a prognostic experiment on the correction of neurodegeneration induced in rats, neuroprotective properties of a steroid hormone estradiol were confirmed and a possible mechanism of the protective effect was suggested. The results allow consideration of both biochemical modifications in protein facilities of a pathology-affected brain and the mechanisms of neurodegeneration and neuroprotection.

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Abbreviations

AD:

Alzheimer’s disease

Aβ:

amyloid beta-peptide

APP:

amyloid precursor protein

DTT:

dithiothreitol

EDTA:

sodium ethylenediaminetetraacetate

PMSF:

phenylmethylsulfonyl fluoride

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Correspondence to L. A. Lysenko.

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Original Russian Text © L.A. Lysenko, N.P. Kantserova, N.L. Rendakov, N.B. Sel’verova, N.N. Nemova, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 5, pp. 572–578.

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Lysenko, L.A., Kantserova, N.P., Rendakov, N.L. et al. Calpain system dysregulation in rat brain at beta-amyloid-induced neurodegeneration. Russ J Bioorg Chem 39, 510–515 (2013). https://doi.org/10.1134/S1068162013050117

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  • DOI: https://doi.org/10.1134/S1068162013050117

Keywords

  • calpain
  • calpastatin
  • calcium
  • neurodegeneration
  • estrogen