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Effect of myelopeptide-1 on the functional activity of phagocytes from mice treated with cyclophosphane

Abstract

The capacity of the bone marrow-derived myelopeptide-1 (MP-1) to affect in vivo and in vitro the functional activity of phagocytes of intact mice and mice treated with a cytostatic agent (cyclophosphane) has been studied. It was found that MP-1 produces a correcting effect on the functional activity of bone marrow and peripheral blood phagocytes. An optimal scheme of the injection of MP-1 to mice with the cyclophosphane-induced immunodeficiency was developed, which provides a maximum immunocorrecting action. MP-1 had the most pronounced effect on the quantitative characteristics and the functional activity of phagocytes of different localization when introduced prior to the cytostatic; under these conditions, the pep tide affects peripheral blood neutrophils. The results obtained enable one to consider MP-1 as a preparation protecting the peripheral blood phagocytes from the damaging action of cyclophosphane.

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Abbreviations

BMC:

bone marrow cells

CL:

chemiluminescence

CY:

cyclophosphane

EDTA:

ethylenediaminetetraacetic acid

PMA:

phorbol myristyl acetate

MP-1:

myelopeptide-1

PS:

physiological solution

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Correspondence to E. A. Kirilina.

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Original Russian Text © E.A. Kirilina, T.B. Masternak, M.A. Efremov, O.Yu. Kamyshnikov, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 5, pp. 579–585.

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Kirilina, E.A., Masternak, T.B., Efremov, M.A. et al. Effect of myelopeptide-1 on the functional activity of phagocytes from mice treated with cyclophosphane. Russ J Bioorg Chem 39, 516–521 (2013). https://doi.org/10.1134/S1068162013050075

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  • DOI: https://doi.org/10.1134/S1068162013050075

Keywords

  • myelopeptide-1 (MP-1)
  • chemiluminescence
  • phagocytes
  • functional activity
  • immunosuppression
  • immunocorrection