Russian Journal of Bioorganic Chemistry

, Volume 39, Issue 4, pp 341–355 | Cite as

Compartmentalization of ROS-mediated signal transduction

  • V. V. Belousov
  • G. N. Enikolopov
  • N. M. MishinaEmail author
Review Article


Localization of signaling molecules close to their targets is the central principle of cell signaling. The colocalization of multicomponent signaling complexes is realized through protein scaffolds that provide better specificity than undirected diffusion of the same components. It has been suggested that ROS-generating complexes follow the principle by specific intracellular localization of ROS production and the limitation of ROS diffusion distances. However, the lack of adequate methods did not allow direct detection of local ROS production to confirm the model of redox signaling compartmentalization. Nevertheless, evidence of local ROS production and the restriction of diffusion were provided by kinetic modeling and data on the subcellular localization of NADPH oxidase isoforms, their adapter proteins, and local restriction of ROS diffusion. Here we shall discuss the properties of antioxidant systems which prevent uncontrolled ROS diffusion from sites of generation to the adjacent subcellular compartments; the current data on the specific localization of NADPH oxidase activity and its influence on intracellular processes; and the recent evidence of the ROS diffusion restriction.


reactive oxygen species hydrogen peroxide compartmentalization NADPH oxidase signal transduction HyPer 



angiotensin receptor type 1




epidermal growth factor


Fönster resonance energy transfer


glutathione/glutathione disulfide


interleukin 1β


interleukin 1 receptor type 1


IκB kinase


IKK kinase

MAPK or MAP-kinase

mitogen-activated protein kinase


myeloid differentiation primary response gene (88)


nicotinamide adenine dinucleotide phosphate


nuclear factor kappa-lightchain-enhancer of activated B cells


NADPH oxidase


regulatory domain of the OxyR transcription factor


platelet-derived growth factor








phosphatase and tensin homologue


protein tyrosine phosphatase 1B


Ras-related C3 botulinum toxin substrate


receptor tyrosine kinase


cell homologue of the Rous sarcoma virus v-Src oncogene


tumor necrosis factor alpha


TNF receptor-associated factor


thioredoxin reduced/oxidized


yellow fluorescent protein


circularly permuted YFP


reactive oxygen species


smooth muscle cells


plasma membrane


endothelial cells


endoplasmic reticulum


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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • V. V. Belousov
    • 1
    • 2
  • G. N. Enikolopov
    • 2
    • 3
  • N. M. Mishina
    • 1
    Email author
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Moscow Institute of Physics and Technology (State University)DolgoprudnyiRussia
  3. 3.Cold Spring Harbor LaboratoryCold Spring HarborUSA

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