Skip to main content

Proteolysis of simple glyprolines by leucine aminopeptidase and enzymes from nasal mucus, brain membranes, and blood of rats

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.


Proteolysis of Pro-Gly-Pro-Leu, Pro-Gly-Pro-Gly and Pro-Gly-Pro compared to Met-Glu-His-Phe-Pro-Gly-Pro (Semax) has been studied. It is shown that all three peptides are significantly more resistant to N-leucine aminopeptidase (EC, Sigma, type VI, 9.2 units/mg), as well as to enzymes of the nasal mucus, the microsomal fraction of brain, and blood of rats. Metabolites of the proteolysis showed that semax derives is His-Phe-Pro-Gly-Pro only; Pro-Gly-Pro-Leu forms Gly-Pro-Leu, Pro-Gly-Pro, and Gly-Pro; Pro-Gly-Pro-Gly gives Pro-Gly-Pro and Gly-Pro; and Pro-Gly-Pro forms Gly-Pro.

This is a preview of subscription content, access via your institution.



membrane enzymes of rat brain


  1. Ashmarin, I.P., Karazeeva, E.P., Lyapina, L.A., and Samonina, G.E., Biochemistry (Moscow), 1998, vol. 63, no. 2, pp. 119–124.

    CAS  Google Scholar 

  2. Ashmarin, I.P., Kamenskii, A.A., Lyapina, L.A., Myasoedov, N.F., and Samonina, G.E., Vopr. Biol. Med. Farm. Khim., 2002, no. 1, pp. 24–27.

    Google Scholar 

  3. Ashmarin, I.P., Lyapina, L.A., Myasoedov, N.F., Pastorova, V.E., and Ul’yanov, A.M., Patent No. 2252778.

  4. Ul’yanov, A.M., Lyapina, L.A., and Shubina, T.A., Vopr. Biol. Med. Farm.. Khim., 2005, no. 4, pp. 19–21.

    Google Scholar 

  5. Ashmarin, I.P., Neurochem. J., 2007, vol. 1, no. 3, pp. 173–175.

    Article  Google Scholar 

  6. Shevchenko, K.V., V’yunova, T.V., Nagaev, I.Yu., Andreeva, L.A., Alfeeva, L.Yu., and Myasoedov, N.F., Russ. J. Bioorg. Chem., 2011, vol. 37, no. 4, pp. 475–482.

    Article  CAS  Google Scholar 

  7. Kopylova, G.N., Umarova, B.A., Samonina, G.E., Guseva, A.A., and Platonova, R.D., Abstracts, in Nauch. Trudy I s”ezda fiziologov SNG, Sochi, Dagomys, 19–21 sentyabrya 2005 (Proc. I Cong. Physiologists of CIS, Sochi, Dagomys, September 19–21, 2005), Moscow: Meditsina-Zdorov’e, 2005, vol. 2, p. 677.

    Google Scholar 

  8. Prockep, D.J., Keiser, H.R., and Sjoerdsma, N.A., Lancet, 1962, vol. 2, pp. 527–528.

    Article  Google Scholar 

  9. Ashmarin, I.P., Alfeeva, L.Yu., Andreeva, L.A., Grivennikov, I.A., Dubynin, V.A., Kamenskii, A.A., Kozlovskaya, M.M., Levitskaya, N.G., Myasoedov, N.F., Nezavibat’ko, V.N., and Seredenin, S.B., Patent No. 2206573.

  10. Lyapina, L.A., Andreeva, L.A., Ul’yanov, A.M., Obergan, T.Yu., Shubina, T.A., Pastorova, V.E., and Myasoedov, N.F., Patent No. 2378005.

  11. Dolotov, O.V., Zolotarev, Yu.A., Dorokhova, E.M., Andreeva, L.A., Alfeeva, L.Yu., Grivennikov, I.A., and Myasoedov, N.F., Russ. J. Bioorg. Chem., 2004, vol. 30, no. 3, pp. 213–217.

    Article  CAS  Google Scholar 

  12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. Biol. Chem., 1951, vol. 193, no. 1, pp. 265–275.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to K. V. Shevchenko.

Additional information

Original Russian Text © K.V. Shevchenko, T.V. V’yunova, I.Yu. Nagaev, L.A. Andreeva, N.F. Myasoedov, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 3, pp. 320–325.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shevchenko, K.V., V’yunova, T.V., Nagaev, I.Y. et al. Proteolysis of simple glyprolines by leucine aminopeptidase and enzymes from nasal mucus, brain membranes, and blood of rats. Russ J Bioorg Chem 39, 283–287 (2013).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Semax
  • glyprolines
  • membranes of rat brain
  • degradation of peptides