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Synthetic conformational antigen which simulates the extracellular part of the M2-muscarinic receptor: interaction with blood sera of patients suffering from idiopathic arrhythmias


Fragments corresponding to the 83–98 sequence of the first extracellular loop and to the 168–192 and 171–182 sequences of the second extracellular loop of the M2-muscarinic receptor (antibodies to this receptor could be markers of early symptoms of heart disorders) were synthesized by solid phase method using the Fmoc-SPPS strategy. A new conformational antigen with the natural location of the disulfide bridge was prepared by selective formation of disulfide bond between the corresponding cysteine residues in the synthe-sized peptides and characterized. The comparative analysis of reactivity of the synthesized peptides towards sera from patients which had no organic heart disease was performed. A new conformational antigen was effectively bound to the sera from patients with idiopathic arrhythmias, but without symptoms of organic heart disease.

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bovine serum albumin

But :











matrix-assisted laser desorption/ionization












N,N,N,N′-tetramethyl-O-(benzotriazole-1-yl)uronium tetrafluoroborate


triisobutyl silane


tri-fluoroacetic acid


high performance liquid chromatog-raphy


enzyme immunoassay


cardiac rhythm disturbance


solid phase peptide synthesis. Other abbreviations used were recommended by IUPAC-IUB


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Correspondence to M. E. Palkeeva.

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Original Russian Text © M.E. Palkeeva, M.V. Sidorova, A.S. Molokoedov, A.A. Az’muko, Zh.D. Bespalova, T.V. Sharf, E.N. Mamochkina, E.E. Efremov, M.M. Rogova, N.A. Mironova, K.A. Zykov, S.P. Golitsyn, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 3, pp. 285–292.

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Palkeeva, M.E., Sidorova, M.V., Molokoedov, A.S. et al. Synthetic conformational antigen which simulates the extracellular part of the M2-muscarinic receptor: interaction with blood sera of patients suffering from idiopathic arrhythmias. Russ J Bioorg Chem 39, 252–258 (2013).

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  • idiopathic arrhythmia
  • antibodies to M2-muscarinic receptor
  • conformational peptide antigens
  • synthetic peptides
  • selective disulfide bond formation
  • solid phase synthesis