Skip to main content
SpringerLink
Log in

Synthetic conformational antigen which simulates the extracellular part of the M2-muscarinic receptor: interaction with blood sera of patients suffering from idiopathic arrhythmias

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

Fragments corresponding to the 83–98 sequence of the first extracellular loop and to the 168–192 and 171–182 sequences of the second extracellular loop of the M2-muscarinic receptor (antibodies to this receptor could be markers of early symptoms of heart disorders) were synthesized by solid phase method using the Fmoc-SPPS strategy. A new conformational antigen with the natural location of the disulfide bridge was prepared by selective formation of disulfide bond between the corresponding cysteine residues in the synthe-sized peptides and characterized. The comparative analysis of reactivity of the synthesized peptides towards sera from patients which had no organic heart disease was performed. A new conformational antigen was effectively bound to the sera from patients with idiopathic arrhythmias, but without symptoms of organic heart disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Boc:

tert-butyloxycarbonyl

BSA:

bovine serum albumin

But :

tert-butyl

DCM:

dichloromethane

DIPEA:

N,N-diisopropylethylamine

Fmoc:

9-fluorenylmethyloxycar-bonyl

HOBT:

1-hydroxybenzotriazole

MALDI:

matrix-assisted laser desorption/ionization

NMP:

N-methylpyrroli-done

MePip:

4-methylpiperidine

PyS:

2-pyridinesulfenyl

ODS:

octadecylsilyl

Pmc:

2,2,5,7,8-pentamethylchroman-6-sulfonyl

TBTU:

N,N,N′,N′-tetramethyl-O-(benzotriazole-1-yl)uronium tetrafluoroborate

TIBS:

triisobutyl silane

TFA:

tri-fluoroacetic acid

HPLC:

high performance liquid chromatog-raphy

EIA:

enzyme immunoassay

CRD:

cardiac rhythm disturbance

SPPS:

solid phase peptide synthesis. Other abbreviations used were recommended by IUPAC-IUB

References

  1. Zheng, Z., Croft, J., Giles, W., and Mensah, G., Circulation, 2001, vol. 104, pp. 2158–2163.

    Article  PubMed  CAS  Google Scholar 

  2. Delacretaz, E., Stevenson, W.G., Ellisio, K.E., Maisel, W.H., and Friedman, P.L., J. Cardiovasc. Electrophysiol, 2000, vol. 11, pp. 11–17.

    Article  PubMed  CAS  Google Scholar 

  3. Magnusson, Y., Wallukat, G., Waagstein, F., Hjalmarson, A., and Hoebeke, J., Circulation, 1994, vol. 89, pp. 2760–2767.

    Article  PubMed  CAS  Google Scholar 

  4. Chugh, S.S., Shen, W.-K., and Luria, D.M., J. Cardiovasc. Electrophysiol., 2000, vol. 11, pp. 328–329.

    Article  PubMed  CAS  Google Scholar 

  5. Kuhn, A., Kottkamp, H., and Thiele, H., Dtsch. Med. Woc, vol. 25, no. 22, pp. 692–697.

  6. Lazzerini, P.E., Capecchi, P.L., and Guideri, F., Basic Research in Cardiol., 2008, vol. 103, pp. 1–11.

    Article  CAS  Google Scholar 

  7. Goin, J.C., Borda, E.S., Auger, S., Storino, R., and Sterin-Borda, L., Heart, 1999, vol. 82, pp. 273–278.

    PubMed  CAS  Google Scholar 

  8. Chiale, P.A., Ferrari, I., Mahler, E., Valazza, M.A., and Elizari, M.V., Circulation, 2001, vol. 103, pp. D. 1765–1771.

    Article  Google Scholar 

  9. Baba, A., Yoshikawa, T., Fukuda, Y., Sugiyama, T., Shimada, M., Akaishi, M., Tsuchimoto, K., Ogawa, S., and Fu, M., Eur. Heart. J, 2004, vol. 25, pp. 1108–1115.

    Article  PubMed  CAS  Google Scholar 

  10. Elies, R., Fu, L.X., Eftekhari, P., Wallukat, G., Schulze, W., Granier, C., Hjalmarson, A., and Hoebeke, J., Eur. J. Biochem., 1998, vol. 251, pp. 659–666.

    Article  PubMed  CAS  Google Scholar 

  11. Fu, L.X., Magnusson, Y., Bergh, C.-H., Liljequist, J., Waagstein, F., Hjalmarson, A., and Hoebeke, J., J. Clin. Invest., 1993, vol. 91, pp. 1964–1968.

    Article  PubMed  CAS  Google Scholar 

  12. Goyal, R.K., N. Engl. J.Med, 1989, vol. 321, pp. 1022–1029.

    Article  PubMed  CAS  Google Scholar 

  13. Johren, K. and Holtje, H.-D., J. Comp.-Aided Mol. Design, 2002, vol. 16, pp. 795–801.

    Article  Google Scholar 

  14. Shulz G.E. and Shirmer R.H., Principles of Protein Structure, New York: Springer-Verlag, 1979.

    Book  Google Scholar 

  15. Han, S.-Y. and Kim, Y.-A., Tetrahedron, 2004, vol. 60, pp. 2447–2467.

    Article  CAS  Google Scholar 

  16. Andreu, D., Albericio, F., Sole, N.A., Munson, M.C., Ferrer, M., and Barany, G., Peptide Synthesis Protocol, Pennington, M.W. and Dunn, B.M., Eds., New Jersey, Totowa: Humana Press, 1994, pp. 91–169.

  17. Sidorova, M.V., Pal’keeva, M.E., Molokoedov, A.S., Az’muko, A.A., Sekridova, A.V., Ovchinnikov, M.V., Levshov, P.A., Afanasieva, O.I., Berestetskaya, Yu.V., Afanasieva, M.I., Rasova, O.A., Bespalova, Zh.D., and Pokrovskii, S.N., Russ. J. Bioorg. Chem., 2009, vol. 35, pp. 285–295.

    Article  CAS  Google Scholar 

  18. Ellman, G.L., Arch. Biochem. Biophys., 1959, vol. 82, pp. 72–77.

    Article  Google Scholar 

  19. Nguyen, D.N., Becker, G.W., and Riggin, R.M., J. Chromatogr. A, 1995, vol. 705, pp. 21–45.

    Article  PubMed  CAS  Google Scholar 

  20. Price, C.P. and Newman, D.J., Principles and Practice of Immunoassay, 2nd ed., London: Macmillan Reference Ltd., 1997.

    Google Scholar 

  21. Atassi, M., Eur. J. Biochem., 1984, vol. 145, pp. 1–20.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Russian Cardiological Research and Production Complex, Russian Ministry of Public Health, ul. 3-ya Cherepkovskaya 15a, Moscow, 121552, Russia

    M. E. Palkeeva, M. V. Sidorova, A. S. Molokoedov, A. A. Az’muko, Zh. D. Bespalova, T. V. Sharf, E. N. Mamochkina, E. E. Efremov, M. M. Rogova, N. A. Mironova, K. A. Zykov & S. P. Golitsyn

Authors
  1. M. E. Palkeeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Sidorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. S. Molokoedov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Az’muko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zh. D. Bespalova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. V. Sharf
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. N. Mamochkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. E. Efremov
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. M. M. Rogova
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. N. A. Mironova
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. K. A. Zykov
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. S. P. Golitsyn
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. E. Palkeeva.

Additional information

Original Russian Text © M.E. Palkeeva, M.V. Sidorova, A.S. Molokoedov, A.A. Az’muko, Zh.D. Bespalova, T.V. Sharf, E.N. Mamochkina, E.E. Efremov, M.M. Rogova, N.A. Mironova, K.A. Zykov, S.P. Golitsyn, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 3, pp. 285–292.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Palkeeva, M.E., Sidorova, M.V., Molokoedov, A.S. et al. Synthetic conformational antigen which simulates the extracellular part of the M2-muscarinic receptor: interaction with blood sera of patients suffering from idiopathic arrhythmias. Russ J Bioorg Chem 39, 252–258 (2013). https://doi.org/10.1134/S1068162013030102

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162013030102

Keywords

Search

Navigation