Abstract
Water-soluble polysaccharide fractions were extracted from the fruit of rowan Sorbus aucuparia L. by water and 0.7% ammonium oxalate water solution. The total yield was 4.2%. It is demonstrated that these fractions are pectin polysaccharides, and their carbon chains are primarily composed of galactunoric acid residue (up to 68%), arabinose and galactose. Sephacryl S-500 gelfiltration of rowan fruit pectin polysaccharides proved their relative homogeneity pertaining to their molecular weights, whereas endo-polygalacturonase enzymatic hydrolysis gives evidence of the presence of extended galacturonan (rhamnogalacturonan) ranges in their carbohydrate chains. Methylation of rowan pectin polysaccharides shows that their carbohydrate pendants are formed by 1,5-linked arabinofuranose residue, 1,4-linked glucopyranose residue, 1,6-linked galactopyranose residue, 1,3,6-linked mannopyranose residue and 1,3,6-linked galactopyranose residue. Glucopyranose residue is identified at non reducible ends of these pendants. It was demonstrated that antioxidant activity of water solutions of pectin polysaccharides extracted from rowan S. aucuparia L. (0.5 mg/mL) is 37–53% of trolox activity, which is 100%.
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Turova, A.D., Lekarstvennye rasteniya SSSR i ikh primenenie (Medicinal Plants of the USSR and Their Use), Moscow, 1974.
Shnaidman, L.O., Kushchinskaya, I.N., Mitel’man, M.K., et al., Rastit. Resur., 1971, vol. 7, no. 1, pp. 68–71.
Usov, A.I., Bilan, M.I., and Klochkova, N.G., Bot. Marina, 1995, vol. 38, no. 3, pp. 43–51.
Wood, P.J. and Siddiqui, I.R., Anal. Biochem., 1971, vol. 39, pp. 418–428.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. Biol. Chem., 1951, vol. 193, pp. 265–275.
York, W.S., Darvill, A.G., McNeil, M.A., Stevenson, T.T., and Albersheim, P., Methods Enzymol., 1985, vol. 118, pp. 3–40.
Kostenko, V.G., Khromatograficheskii analiz sakharov, poluchaemykh v protsesse pererabotki rastitel’nogo syr’ya (Chromatographic Analysis of Saccharides Obtained during Processing of Raw Plant Materials), Moscow, 1984.
Charms, S., Fishbein, L., Vagman, J., Weinstein, M., Cowling, G., Dolphin, D., Harborne, J., Lederer, M., Yanak, E., and Allard, E., Khromatografiya. Prakticheskoe prilozhenie metoda (Chromatography: Practical Application of the Method), Moscow, 1986, vol. 2.
Hakomori, S., J. Biochem., 1964, vol. 55, no. 2, pp. 205–208.
Ovodova, R.G., Bushneva, O.A., Golovchenko, V.V., Popov, S.V., and Ovodov, Yu.S., RF Patent No. 2149642, Byull. Izobret., 2000, no. 15.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebera, P.A., and Smith, F., Anal. Chem., 1956, vol. 28, no. 2, pp. 350–356.
Yang, S.S., Cheng, K.T., Lin, Y.S., Liu, Y.W., and Hou, W.C., J. Agric. Food Chem., 2004, vol. 52, pp. 4270–4273.
Zlobin, A.A. and Ovodova, R.G., Rastit. Resur., 2007, vol. 43, no. 2, pp. 52–59.
Ovdov, Yu.S., Bioorg. Khim., 1998, vol. 24, no. 7, pp. 483–501.
Gracheva, I.M., Tekhnologiya fermentnykh prerparatov (Technology of Enzyme Preparations), Moscow, 2000.
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Original Russian Text © A.A. Zlobin, E.A. Ìartinson, S.G. Litvinets, I.A. Ovechkina, E.A. Durnev, R.G. Ovodova, 2011, published in Khimija Rastitel’nogo Syr’ja, 2011, No. 1, pp. 39–44.
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Zlobin, A.A., Martinson, E.A., Litvinets, S.G. et al. Pectin polysaccharides of rowan Sorbus aucuparia L.. Russ J Bioorg Chem 38, 702–706 (2012). https://doi.org/10.1134/S1068162012070242
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DOI: https://doi.org/10.1134/S1068162012070242