Abstract
A simple approach to the synthesis of amidophosphite synthons of achiral non-nucleotide inserts using 4-(2-(4,4′-dimethoxytrityloxy)ethyl)morpholine-2,3-dione as a backbone of key precursor was suggested. Non-nucleotide synthons were synthesized using this approach that were suitable for synthesis of acridine-containing oligonucleotide derivatives, as well as oligonucleotides with branched carbohydrate-phosphate backbone.
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Abbreviations
- TBDMS:
-
tret-butyldimethylsilyl
- DMTr:
-
dimethoxytrityl
- CEP:
-
2-cyanoethoxy diisopropylamino phosphinyl
- Lev:
-
levulinyl
- DIPEA:
-
diisopropylethylamine
- TEA:
-
triethylamine
- DMAP:
-
4-N,N-dimethylaminopyridine
References
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Original Russian Text © M.S. Kupryushkin, D.V. Pyshnyi, 2012, published in Bioorganicheskaya Khimiya, 2012, Vol. 38, No. 6, pp. 745–750.
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Kupryushkin, M.S., Pyshnyi, D.V. A convenient method for the synthesis of phosphoramidite non-nucleotide inserts for preparation of functionalized oligonucleotides. Russ J Bioorg Chem 38, 662–666 (2012). https://doi.org/10.1134/S1068162012060106
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DOI: https://doi.org/10.1134/S1068162012060106