Abstract
A novel endogenous inhibitor from the hepatopancreas of the Kamchatka crab Paralithodes camtschatica has been isolated by affinity chromatography on gramicidin C-diasorb followed by gel filtration on Sephadex G-100. The inhibitor is a protein with M of 66 kDa. It has an optimum of activity at 15–20°C, is stable in the range of 4–40°C, and is completely inactivated upon heating to 50°C and above. For the manifestation of the inhibitory effect of the protein, the presence of NaCl in buffer at a concentration of 0.9–20% is necessary. The inhibitor slows down the spreading of cells in vitro. The effectiveness of the inhibition of cell spreading depends on the cell type and probably the degree of malignization: the effect is most clearly pronounced in fibroblasts (normal and transformed), is less marked in epithelial cells, and is not pronounced in fibrosarcoma cells.
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Abbreviations
- pNA,p :
-
nitroanilide
- Glp:
-
pyroglutamyl
References
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Original Russian Text © S.A. Kuznetsov, S.A. Golyshev, Yu.A. Rudenskaya, V.A. Isaev, I.P. Kuranova, G.N. Rudenskaya, 2012, published in Bioorganicheskaya Khimiya, 2012, Vol. 38, No. 3, pp. 333–341.
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Kuznetsov, S.A., Golyshev, S.A., Rudenskaya, Y.A. et al. A novel endogenous inhibitor from the hepatopancreas of the Kamchatka crab Paralithodes camtschaticus . Russ J Bioorg Chem 38, 290–297 (2012). https://doi.org/10.1134/S1068162012020069
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DOI: https://doi.org/10.1134/S1068162012020069