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Monoclonal antibodies labeled with colloidal gold for immunochromatographic express analysis of diphtheria toxin

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Abstract

A one-step immunochromatographic method for the detection of the diphtheria toxin in different water samples (phosphate buffer, milk, and human nasopharyngeal swab) was developed using a conjugate of monoclonal antibodies labeled with colloidal gold. The detection limit of diphtheria toxin was 10 ng/ml, the time of the analysis was 15 min. The use of silver to enhance the reaction sensitivity and scanning equipment to evaluate the immunochromatography results decreased the detection limit to 1.25 ng/ml.

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Abbreviations

DT:

diphtheria toxin

SEA, SEB, SED, SECI, SEE, and TSST:

staphylococcal enterotoxins A, B, D, C1, E, and staphylococcal toxin causing tissue shock

CT:

cholera toxin

LT:

thermolabile toxin of E. coli

MA:

monoclonal antibodies

PBS:

phosphate-saline buffer, pH 7.4

PBST:

PBS with 0.1% Tween-20

STR-HRP:

streptavidin-horseradish peroxidase conjugate

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, B-437, 117997, Russia

    I. A. Lyubavina, T. I. Valyakina & E. V. Grishin

Authors
  1. I. A. Lyubavina
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  2. T. I. Valyakina
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  3. E. V. Grishin
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Correspondence to I. A. Lyubavina.

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Original Russian Text © I.A. Lyubavina, T.I. Valyakina, E.V. Grishin, 2011, published in Bioorganicheskaya Khimiya, 2011, Vol. 37, No. 3, pp. 366–373.

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Lyubavina, I.A., Valyakina, T.I. & Grishin, E.V. Monoclonal antibodies labeled with colloidal gold for immunochromatographic express analysis of diphtheria toxin. Russ J Bioorg Chem 37, 326–332 (2011). https://doi.org/10.1134/S1068162011030113

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