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Differentiation of α-hemolytic streptococci by direct mass spectrometric profiling

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Abstract

Modern phenotypic and genetic methods (with the exception of multilocus sequence typing) do not provide a reliable differentiation of α-hemolytic streptococci of the Mitis group. In this study, we obtained MALDI mass spectra for 28 clinical isolates that were initially identified by standard methods as Streptococcus pheumoniae. The isolate distribution that was obtained in the course of clustering correlated well with the data on multilocus typing. These studied isolates were attributed by this typing to two closely related species: S. pneumoniae and S. mitis. A classification model that differentiated α-hemolytic streptococci with 100% sensitivity and 94.6% specificity was developed on the basis of a genetic algorithm. Two peaks that were different for the S. mitis and S. pneumoniae isolates were revealed by the analysis of statistical diagrams of peak areas.

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Abbreviations

α-CHCA:

α-cyano-4-hydroxycinnamic acid

MALDI:

matrix-assisted laser desorbtion/ionization

MLST:

multilocus sequence typing

ST:

sequence type

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Authors and Affiliations

  1. Research Institute of Physicochemical Medicine, ul. Malaya Pirogovskaya 1a, Moscow, 119435, Russia

    A. D. Borovskaya, E. N. Il’ina & V. M. Govorun

  2. National Agency for Clinical Pharmacology and Pharmacy, Moscow, Russia

    T. A. Savinova & S. V. Sidorenko

  3. Research Institute of Children Infection Diseases, St. Petersburg, Russia

    S. V. Sidorenko

  4. National Research Center of Antibiotics, Moscow, Russia

    S. A. Grudinina

  5. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997, Russia

    V. M. Govorun

Authors
  1. A. D. Borovskaya
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  2. E. N. Il’ina
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  3. T. A. Savinova
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  4. S. V. Sidorenko
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  5. S. A. Grudinina
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  6. V. M. Govorun
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Corresponding author

Correspondence to E. N. Il’ina.

Additional information

Original Russian Text © A.D. Borovskaya, E.N. Il’ina, T.A. Savinova, S.V. Sidorenko, S.A. Grudinina, V.M. Govorun, 2011, published in Bioorganicheskaya Khimiya, 2011, Vol. 37, No. 1, pp. 61–69.

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Borovskaya, A.D., Il’ina, E.N., Savinova, T.A. et al. Differentiation of α-hemolytic streptococci by direct mass spectrometric profiling. Russ J Bioorg Chem 37, 53–60 (2011). https://doi.org/10.1134/S1068162011010043

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  • DOI: https://doi.org/10.1134/S1068162011010043

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