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Oligonucleotide microarray for the subtyping of influenza virus a neuraminidase

Abstract

A microarray for the subtyping of influenza A neuraminidase is presented. The selection of oligoprobes proceeded in two steps. The first step included the selection of peptides specific for each subtype of neuraminidase. At the second step, the oligoprobes were calculated using the found peptide structures with the subsequent additional selection of the most specific and representative probes. From 19 to 24 probes were used for the determination of each neuraminidase subtype. The microarray testing for 19 samples with the most widespread types (N1 and N2) specifies in an unequivocal definition 18 of them and only 1 isolate has not been identified.

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Abbreviations

SSC:

0.15 M NaCl, 0.015 M sodium citrate, pH 7.0

Cy3:

fluorescent cyanic dyestuff

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Correspondence to V. A. Ryabinin.

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Original Russian Text © V.A. Ryabinin, E.V. Kostina, A.A. Neverov, G.A. Maksakova, A.N. Sinyakov, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 5, pp. 688–698.

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Ryabinin, V.A., Kostina, E.V., Neverov, A.A. et al. Oligonucleotide microarray for the subtyping of influenza virus a neuraminidase. Russ J Bioorg Chem 36, 634–644 (2010). https://doi.org/10.1134/S1068162010050122

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  • DOI: https://doi.org/10.1134/S1068162010050122

Key words

  • influenza virus A
  • neuraminidase
  • microarray
  • typing
  • diagnostics