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N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligoribonucleotides


An effective modification of the phosphotriester method has been developed for the automatic synthesis of DNA and RNA fragments using O-nucleophilic intramolecular catalysis and the 2-(azidomethyl)benzoyl group for the protection of the amino groups of nucleotide heterocyclic bases.

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triethyl-ammonium bicarbonate








tetrabutylammonium fluoride


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Correspondence to V. A. Efimov.

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Original Russian Text © V.A. Efimov, A.V. Aralov, S.A. Grachev, O.G. Chakhmakhcheva, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 5, pp. 681–687.

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Efimov, V.A., Aralov, A.V., Grachev, S.A. et al. N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligoribonucleotides. Russ J Bioorg Chem 36, 628–633 (2010).

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Key words

  • 2-azidomethylbenzoyl group
  • intramolecular catalysis
  • oligonucleotides synthesis
  • phosphotriester method