Abstract
An effective modification of the phosphotriester method has been developed for the automatic synthesis of DNA and RNA fragments using O-nucleophilic intramolecular catalysis and the 2-(azidomethyl)benzoyl group for the protection of the amino groups of nucleotide heterocyclic bases.
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Abbreviations
- AZM:
-
azidomethyl
- AZMB:
-
o-azidomethylben-zoyl
- DBU:
-
1,8-diazabicyclo[5.4.0]undec-7-ene
- DMTr:
-
4,4′-dimethoxytrityl
- NBS:
-
2-nitrobenzenesulfenyl
- TEAB:
-
triethyl-ammonium bicarbonate
- TMS:
-
trimethylsilyl
- TPS:
-
2,4,6-tri-isopropylbenzenesulfonyl
- TIPDS-C12 :
-
1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane
- TBAF:
-
tetrabutylammonium fluoride
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Original Russian Text © V.A. Efimov, A.V. Aralov, S.A. Grachev, O.G. Chakhmakhcheva, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 5, pp. 681–687.
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Efimov, V.A., Aralov, A.V., Grachev, S.A. et al. N-azidomethylbenzoyl blocking group in the phosphotriester synthesis of oligoribonucleotides. Russ J Bioorg Chem 36, 628–633 (2010). https://doi.org/10.1134/S1068162010050110
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DOI: https://doi.org/10.1134/S1068162010050110