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The structure of the O-specific polysaccharide from a mutant of nitrogen-fixing rhizobacterium Azospirillum brasilense Sp245 with an altered plasmid content

Abstract

The rhizobacteria Azospirillum brasilense Sp245 produce immunochemically different lipopolysaccharides LPSI and LPSII, both containing identical pentasaccharides built from D-rhamnose residues as the repeating units of O-specific polysaccharides (OPS). In this study, we report the structure of the OPS from A. brasilense LPSILPSII mutant Sp245.5, which spontaneously lost the p85 and p120 plasmids upon the formation of a new 300-MDa megaplasmid after the long-term storage of the bacteria in a rich medium. The repeating unit of the OPS of A. brasilense Sp245.5 appeared to be a disaccharide consisting of residues of N-acetyl-D-galactosamine and N-acetyl-D-mannosaminuronic acid:

$$ \to 6) - \alpha - D - GalpNAc - (1 \to 4) - \beta - D - ManpNAcA - (1 \to $$

.

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Abbreviations

CBPS:

Calcofluor-binding polysaccharides

CC:

coupling constant

COSY:

correlation spectroscopy

CPS:

capsular polysaccharides

EPS:

exopolysaccharides

HMBS:

heteronuclear multiple-bond correlation spectroscopy

HSQC:

heteronuclear single-quantum coherence spectroscopy

LPS:

lipopolysaccharide

OPS:

O-specific polysaccharide

ROESY:

rotating-frame nuclear Overhauser enhancement spectroscopy

TOCSY:

total correlation spectroscopy

References

  1. Schnaitman, C.A. and Klena, J.D., Microbiol. Rev., 1993, vol. 57, pp. 655–682.

    CAS  PubMed  Google Scholar 

  2. Del Gallo, M., Negi, M., and Neyra, C.A., J. Bacteriol., 1989, vol. 171, pp. 3504–3510.

    PubMed  Google Scholar 

  3. Vanbleu, E., Choudhury, B.P., Carlson, R.W., and Vanderleyden, J., Environ. Microbiol., 2005, vol. 7, pp. 1769–1774.

    Article  CAS  PubMed  Google Scholar 

  4. Vanbleu, E., Marchal, K., Lambrecht, M., Mathys, J., and Vanderleyden, J., FEMS Microbiol. Letts., 2004, vol. 232, pp. 165–172.

    Article  CAS  Google Scholar 

  5. Jofre, E., Lagares, A., and Mori, G., FEMS Microbiol. Letts., 2004, vol. 231, pp. 267–275.

    Article  CAS  Google Scholar 

  6. Fedonenko, Y.P., Zatonsky, G.V., Konnova, S.A., Zdorovenko, E.L., and Ignatov, V.V., Carbohydr. Res., 2002, vol. 337, pp. 869–872.

    Article  CAS  PubMed  Google Scholar 

  7. Katzy, E.I., Matora, L.Y., Serebrennikova, O.B., and Scheludko, A.V., Plasmid, 1998, vol. 40, pp. 73–83.

    Article  CAS  PubMed  Google Scholar 

  8. Fedonenko, Yu.P., Zdorovenko, E.L., Konnova, S.A., Ignatov, V.V., and Shlyakhtin, G.V., Mikrobiologiya, 2004, vol. 73, pp. 180–187.

    Google Scholar 

  9. Katsy, E.I., Borisov, I.V., Petrova, L.P., and Matora, L.Yu., Genetika, 2002, vol. 38, pp. 182–189.

    CAS  PubMed  Google Scholar 

  10. Katsy, E.I., Borisov, I.V., Mashkina, A.B., and Panasenko, V.I., Mol. Genet., 1994, no. 2, pp. 29–34.

  11. Bock, K. and Pedersen, C., Adv. Carbohydr. Chem. Biochem., 1983, vol. 4, pp. 127–166.

    Google Scholar 

  12. Lipkind, G.M., Shashkov, A.S., Knirel, Y.A., Vinogradov, E.V., and Kochetkov, N.K., Carbohydr. Res., 1988, vol. 175, pp. 59–75.

    Article  CAS  PubMed  Google Scholar 

  13. Shashkov, A.S., Lipkind, G.M., Knirel, Y.A., and Kochetkov, N.K., Magn. Reson. Chem., 1988, vol. 26, pp. 735–747.

    Article  CAS  Google Scholar 

  14. Lugowski, C., Romanowska, E., Kenne, L., and Lindberg, B., Carbohydr. Res., 1983, vol. 118, pp. 173–181.

    Article  CAS  Google Scholar 

  15. Leone, S., Lanzetta, R., Scognamiglio, R., Alfieri, F., Izzo, V., Di Donato, A., Parrilli, M., Holst, O., and Molinaro, A., Carbohydr. Res., 2008, vol. 343, pp. 674–684.

    Article  CAS  PubMed  Google Scholar 

  16. Leone, S., Izzo, V., Lanzetta, R., Molinaro, A., Parrilli, M., and Di Donato, A., Carbohydr. Res., 2005, vol. 340, pp. 651–656.

    Article  CAS  PubMed  Google Scholar 

  17. Westphal, O. and Jann, K., Methods Carbohydr. Chem., 1965, vol. 5, pp. 83–91.

    CAS  Google Scholar 

  18. Konnova, S.A., Makarov, O.E., Skvortsov, I.M., and Ignatov, V.V., FEMS Microbiol. Letts., 1994, vol. 118, pp. 93–99.

    Article  CAS  Google Scholar 

  19. Sawardeker, J.S., Sloneker, J.H., and Jeanes, A., Anal. Chem., 1965, vol. 37, pp. 1602–1603.

    Article  CAS  Google Scholar 

  20. Conrad, H.E., Methods Carbohydr. Chem., 1972, vol. 6, pp. 361–364.

    CAS  Google Scholar 

Download references

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Correspondence to Yu. P. Fedonenko.

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Original Russian Text © Yu.P. Fedonenko, E.I. Katsy, L.P. Petrova, A.S. Boyko, E.L. Zdorovenko, V.V. Kachala, A.S. Shashkov, Yu.A. Knirel, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 2, pp. 236–240.

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Fedonenko, Y.P., Katsy, E.I., Petrova, L.P. et al. The structure of the O-specific polysaccharide from a mutant of nitrogen-fixing rhizobacterium Azospirillum brasilense Sp245 with an altered plasmid content. Russ J Bioorg Chem 36, 219–223 (2010). https://doi.org/10.1134/S1068162010020111

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  • DOI: https://doi.org/10.1134/S1068162010020111

Key words

  • Azospirillum brasilense
  • O-specific polysaccharide structure
  • plasmid rearrangements