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The oligoclonal expansion of T cells: The investigation of its stability over time

Abstract

A novel experimental approach to the investigation of the repertoire of peripheral T lymphocytes of patients suffering from ankylosing spondylitis (AS) is proposed. This approach is based on the wide-range sequencing of cDNA of the β-chain of the T-cellular receptor (TcR). The results of the analysis of the diversity of sequences of the TcR antigen-binding domain (CDR3) inside the total pool of one patient with AS are presented by the example of the second V family (BV2) of TcR. The expansion of six independent TcR-expressing clones of T cells with a similar amino acid sequence of the CDR3 domains was proposed based on the results of the comparative structural analysis of the clone libraries of the cDNA of TcR BV2. The long-time stable expansion of these T clones was demonstrated during the development of the disease by specific monitoring.

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Abbreviations

AS:

ankylosing spondylitis (Bekhterev’s disease)

B-TcR:

β-chain of T-cellular receptor

BV, BD, BJ, and BC:

the main structural domains of the β-chain of TcR

BV2:

the second TcR family (classification according to the β-chain)

CDR3:

antigen-binding complementarity-determining region of TcR

RT-PCR:

polymerase chain reaction with reversed transcription

TcR:

T-cellular receptor

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Correspondence to A. V. Chkalina.

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Original Russian Text © A.V. Chkalina, I.V. Zvyagin, I.Z. Mamedov, O.V. Britanova, D.B. Staroverov, Yu.B. Lebedev, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 2, pp. 206–214.

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Chkalina, A.V., Zvyagin, I.V., Mamedov, I.Z. et al. The oligoclonal expansion of T cells: The investigation of its stability over time. Russ J Bioorg Chem 36, 191–198 (2010). https://doi.org/10.1134/S1068162010020081

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  • DOI: https://doi.org/10.1134/S1068162010020081

Key words

  • repertoire of T lymphocytes
  • oligoclonal T-cellular expansion
  • genes of the T-cellular receptor
  • ankylosing spondylitis