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New azobenzene derivatives for directed modification of proteins

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Abstract

Derivatives of azobenzene which contained a maleimide group in one of the benzene rings (for binding to a protein cysteine residue) and maleimide, hydroxyl, or carboxyl substitutes in another benzene ring were synthesized. The reactivity of these compounds towards a cysteine residue of a protein and their optical properties in a free form and after their attachment to the mutant forms of the SsoII restriction endonuclease were studied.

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Abbreviations

NBS:

N-bromosuccinimide

PEG-Mal:

(methylpolyethylenglycol12)3-polyethylenglycol4-maleimide

R.SsoII:

the SsoII restriction endonuclease

SDS:

sodium dodecylsulfate

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Authors and Affiliations

  1. Chemistry Department, Faculty of Bioengineering and Bioinformatics, A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia

    Le Thi Hien, A. Yu. Ryazanova, T. S. Zatsepin, E. M. Volkov, E. A. Kubareva, T. I. Velichko & T. S. Oretskaya

  2. Institute of Biochemistry, Justus-Liebig University, Giessen, Germany

    B. Schierling & A. Pingoud

Authors
  1. Le Thi Hien
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  2. B. Schierling
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  3. A. Yu. Ryazanova
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  4. T. S. Zatsepin
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  5. E. M. Volkov
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  6. E. A. Kubareva
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  7. T. I. Velichko
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  8. A. Pingoud
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  9. T. S. Oretskaya
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Corresponding author

Correspondence to T. S. Oretskaya.

Additional information

Original Russian Text © Le Thi Hien, B. Schierling, A.Yu. Ryazanova, T.S. Zatsepin, E.M. Volkov, E.A. Kubareva, T.I. Velichko, A. Pingoud, T.S. Oretskaya, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 5, pp.610–617.

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Hien, L.T., Schierling, B., Ryazanova, A.Y. et al. New azobenzene derivatives for directed modification of proteins. Russ J Bioorg Chem 35, 549–555 (2009). https://doi.org/10.1134/S1068162009050033

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  • DOI: https://doi.org/10.1134/S1068162009050033

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