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Functional interaction of the RNA polymerase II Rpb11 subunit with the subunit Med18 (Srb5) of the Saccharomyces cerevisiae mediator complex

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Abstract

The SRB5 gene encoding the Med18 (Srb5) subunit of the Mediator complex of the Saccharomyces cerevisiae transcription apparatus was identified as a multicopy suppressor of the Leu111Ala (L111A) point mutation in the C-terminal region of the yeast RNA polymerase II subunit Rpb11. Thus, the functional interaction between one of the Mediator components and the core of the major transcription enzyme was shown for the first time. It is also important that the suppressed point mutation is located in the short C-terminal region of the Rpb11 subunit, which, as was demonstrated in our previous studies, has played an essential role in the evolution of the eukaryotic transcription machinery.

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References

  1. Thompson, C.M., Koleske, A.J., Chao, D.M., and Young, R.A., Cell, 1993, vol. 73, pp. 1361–1375.

    Article  PubMed  CAS  Google Scholar 

  2. Kim, Y.-J., Bjorklund, S., Li, Y., Sayre, M.H., and Kornberg, R.D., Cell, 1994, vol. 77, pp. 599–608.

    Article  PubMed  CAS  Google Scholar 

  3. Kornberg, R.D., Trends Biochem. Sci., 2005, vol. 30, pp. 235–239.

    Article  PubMed  CAS  Google Scholar 

  4. Blazek, E., Mittler, G., and Meisterernst, M., Chromosoma, 2005, vol. 113, pp. 399–408.

    Article  PubMed  CAS  Google Scholar 

  5. Asturias, F.J., Jiang, Y.W., Myers, L.C., Gustafsson, C.M., and Kornberg, R.D., Science, 1999, vol. 283, pp. 985–987.

    Article  PubMed  CAS  Google Scholar 

  6. Davis, J., Takagi, Y., Kornberg, R.D., and Asturias, F.J., Mol. Cell, 2002, vol. 10, pp. 409–415.

    Article  PubMed  CAS  Google Scholar 

  7. Bourbon, H.M., Aguilera, A., Ansari, A.Z., Asturias, F.J., Berk, A.J., et al., Mol. Cell, 2004, vol. 14, pp. 553–557.

    Article  PubMed  CAS  Google Scholar 

  8. Conaway, R.C., Sato, S., Tomomori-Sato, C., Yao, T., and Conaway, J.W., Trends Biochem. Sci., 2005, vol. 30, pp. 250–255.

    Article  PubMed  CAS  Google Scholar 

  9. Armache, K.-J., Kettenberger, H., and Cramer, P., Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 6964–6968.

    Article  PubMed  CAS  Google Scholar 

  10. Westover, K.D., Bushnell, D.A., and Kornberg, R.D., Cell, 2004, vol. 119, pp. 481–489.

    Article  PubMed  CAS  Google Scholar 

  11. Kettenberger, H., Armache, K.J., and Cramer, P., Mol. Cell, 2004, vol. 16, pp. 955–965.

    Article  PubMed  CAS  Google Scholar 

  12. Kornberg, R.D., Proc. Natl. Acad. Sci. USA, 2007, vol. 104, pp. 12955–12961.

    Article  PubMed  CAS  Google Scholar 

  13. Takagi, Y. and Kornberg, R.D., J. Biol. Chem., 2006, vol. 281, pp. 80–89.

    Article  PubMed  CAS  Google Scholar 

  14. Chadick, J.Z. and Asturias, F.J., Trends Biochem. Sci., 2005, vol. 30, pp. 264–271.

    Article  PubMed  CAS  Google Scholar 

  15. Myers, L.C., Gustafsson, C.M., Bushnell, D.A., Lui, M., Erdjument-Bromage, H., Tempst, P., and Kornberg, R.D., Genes Dev., 1998, vol. 12, pp. 45–54.

    Article  PubMed  CAS  Google Scholar 

  16. Sobennikova, M.V., Shematorova, E.K., and Shpakovskii, G.V., Mol. Biol., 2007, vol. 41, pp. 433–449.

    Article  CAS  Google Scholar 

  17. Egloff, S. and Murphy, S., Trends Genet., 2008, vol. 24, pp. 280–288.

    Article  PubMed  CAS  Google Scholar 

  18. Malik, S. and Roeder, R.G., Trends Biochem. Sci., 2005, vol. 30, pp. 256–263.

    Article  PubMed  CAS  Google Scholar 

  19. Benga, W.J., Grandemange, S., Shpakovski, G.V., Shematorova, E.K., Kedinger, C., and Vigneron, M., Nucleic Acids Res., 2005, vol. 33, pp. 3582–3590.

    Article  PubMed  CAS  Google Scholar 

  20. Stettler, S., Chiannilkulchai, N., Hermann-Le, Denmat S., Lalo, D., Lacroute, F., Sentenac, A., and Thuriaux, P., Mol. Gen. Genet., 1993, vol. 239, pp. 169–176.

    PubMed  CAS  Google Scholar 

  21. Shpakovskii, G.V., Shematorova, E.K., Proshkin, S.A., Shpakovskii, D.G., Timchenko, K.A., and Proshkina, G.M., Tez. dokl. VII chtenii, posvyashchennykh pamyati akademika Yu.A. Ovchinnikova (Abstracts of Papers, VII Lecture in Memory of Academician Yu.A. Ovchinnikov), Moscow-Pushchino, October 4–7, 2004, Poligraf. Uch. Inst. Bioorg. Khim. Ros. Akad. Nauk, 2004, pp. 11–12.

  22. Vinciguerra, P. and Stutz, F., Curr. Opin. Cell. Biol., 2004, vol. 16, pp. 285–292.

    Article  PubMed  CAS  Google Scholar 

  23. Balciunas, D. and Ronne, H., Mol. Gen. Genet., 1999, vol. 262, pp. 589–599.

    Article  PubMed  CAS  Google Scholar 

  24. Samuelsen, C.O., Barazhenok, V., Spahr, H., Kieselbach, T., Holmberg, S., and Gustafsson, C.M., Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 6422–6427.

    Article  PubMed  CAS  Google Scholar 

  25. Elmlund, H., Baraznenok, V., Lindahl, M., Samuelsen, C.O., Koeck, P.J.B., Holmberg, S., Hebert, H., and Gustafsson, C.M., Proc. Natl. Acad. Sci. USA, 2006, vol. 103, pp. 15788–15793.

    Article  PubMed  CAS  Google Scholar 

  26. Becker, D.M., Fikes, J.D., and Guarente, L., Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 1968–1972.

    Article  PubMed  CAS  Google Scholar 

  27. Linder, T., Zhu, X., Barazhenok, V., and Gustafsson, C.M., Biochem. Biophys. Res. Commun., 2006, vol. 349, pp. 948–953.

    Article  PubMed  CAS  Google Scholar 

  28. Shpakovski, D.G., Shematorova, E.K., and Shpakovski, G.V., Bioorg. Khim., 2004, vol. 30, pp. 621–625 [Russ. J. Bioorgan. Chemistry, 2004, vol. 30, pp. 561–565].

    Google Scholar 

  29. Liu, Y., Ranish, J.A., Aebersold, R., and Hahn, S., J. Biol. Chem., 2001, vol. 276, pp. 7169–7175.

    PubMed  CAS  Google Scholar 

  30. Tardiff, D.F., Abruzzi, K.C., and Rosbash, M., Proc. Natl. Acad. Sci. USA, 2007, vol. 104, pp. 19948–19953.

    Article  PubMed  CAS  Google Scholar 

  31. Takagi, Y., Calero, G., Komori, H., Brown, J.A., Ehrensberger, A.H., Hudmon, A., Asturias, F., and Kornberg, R.D., Mol. Cell, 2006, vol. 23, pp. 355–364.

    Article  PubMed  CAS  Google Scholar 

  32. Lariviére, L., Seizl, M., van Wageningen, S., Röther, S., van de Pasch, L., Feldmann, H., Strässer, K., Hahn, S., Holstege, F.C., and Cramer, P., Genes Dev., 2008, vol. 22, pp. 872–877.

    Article  PubMed  Google Scholar 

  33. Guglielmi, B., van Berkum, N.L., Klapholz, B., Bijma, T., Boube, M., Boschiero, C., Bourbon, H.M., Holstege, F.C.P., and Werner, M., Nucleic Acids Res., 2004, vol. 32, pp. 5379–5391.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    G. M. Proshkina & G. V. Shpakovskii

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  1. G. M. Proshkina
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  2. G. V. Shpakovskii
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Correspondence to G. V. Shpakovskii.

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Original Russian Text © G.M. Proshkina, G.V. Shpakovskii, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 4, pp. 572–576.

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Proshkina, G.M., Shpakovskii, G.V. Functional interaction of the RNA polymerase II Rpb11 subunit with the subunit Med18 (Srb5) of the Saccharomyces cerevisiae mediator complex. Russ J Bioorg Chem 35, 518–522 (2009). https://doi.org/10.1134/S1068162009040153

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  • DOI: https://doi.org/10.1134/S1068162009040153

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