Skip to main content

Bacterial antigen polysaccharides. 43. Structure for the O-specific polysaccharide of Providencia alcalifaciens O46


Acidic O-specific polysaccharide containing D-glucose, D-glucuronic acid, L-fucose, and 2-acetamido-2-deoxy-D-glucose was obtained by mild acid degradation of lipopolysaccharide from Providencia alcalifaciens O46. The following structure of the hexasaccharide repeating unit of the O-specific polysaccharide was established using methylation analysis along with 1H and 13C NMR spectroscopy, including 2D 1H, 1H-COSY, TOCSY, ROESY, 1H, 13C-HSQC, and HMQC-TOCSY experiments:

This is a preview of subscription content, access via your institution.





O-specific polysaccharide


O-deacetylated polysaccharide


correlation spectroscopy


heteronuclear multiple-quantum/singe-quantum coherence spectroscopy




rotational frame nuclear Overhauser effect spectroscopy


total correlation spectroscopy


  1. Perepelov, A.V., Senchenkova, S.N., Shashkov, A.S., Knirel, Y.A., Liu, B., Feng, L., and Wang, L., Bioorg. Khim., 2008, vol. 34, pp. 808–812 [Russ. J. Bioorg. Chem. (Engl. Transl.), vol. 34, pp. 725–729].

    PubMed  CAS  Google Scholar 

  2. O’Hara, C.M., Brenner, F.W., and Miller, J.M., Clin. Microbiol. Rev., 2000, vol. 13, pp. 534–546.

    PubMed  Article  Google Scholar 

  3. Somvanshi, V.S., Lang, E., Straubler, B., Sproer, C., Schumann, P., Ganguly, S., Saxena, A.K., and Stackebrandt, E., Int. J. Syst. Evol. Microbiol., 2006, vol. 56, pp. 629–633.

    PubMed  Article  CAS  Google Scholar 

  4. Ewing, W.H, in Identification of Enterobacteriaceae, Edwards, P.R., Ed., New York: Elsevier, 1986, pp. 454–459.

    Google Scholar 

  5. Kocharova, N.A., Kondakova, A.N., Vinogradov, E., Ovchinnikova, O.G., Lindner, B., Shashkov, A.S., Rozalski, A., and Knirel, Y.A., Chem. Eur. J., 2008, vol. 14, pp. 6184–6191.

    Article  CAS  Google Scholar 

  6. Westphal, O. and Jann, K., Methods Carbohydr. Chem., 1965, vol. 5, pp. 83–91.

    CAS  Google Scholar 

  7. Duus, J.O., Gotfredsen, C.H., and Bock, K., Chem. Rev., 2000, vol. 100, pp. 4589–4614.

    PubMed  Article  CAS  Google Scholar 

  8. Jansson, P.-E., Kenne, L., and Widmalm, G., Carbohydr. Res., 1989, vol. 188, pp. 169–191.

    PubMed  Article  CAS  Google Scholar 

  9. Lipkind G.M., Shashkov A.S., Knirel Y.A., Vinogradov E.V., Kochetkov N.K., Carbohydr. Res., 1988, vol. 175, pp. 59–75.

    PubMed  Article  CAS  Google Scholar 

  10. Hakomori, S., J. Biochem.,(Tokyo), 1964, vol. 55, pp. 205–208.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to O. G. Ovchinnikova.

Additional information

Original Russian Text © O.G. Ovchinnikova, N.A. Kocharova, A.S. Shashkov, Yu.A. Knirel, A. Rozalski, 2009, published in Bioorganicheskaya Khimiya, 2009, vol. 35, No. 3, pp. 408–413.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ovchinnikova, O.G., Kocharova, N.A., Shashkov, A.S. et al. Bacterial antigen polysaccharides. 43. Structure for the O-specific polysaccharide of Providencia alcalifaciens O46. Russ J Bioorg Chem 35, 370–375 (2009).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

Key words

  • Providencia alcalifaciens
  • O-antigen
  • O-specific polysaccharide
  • structure
  • NMR spectroscopy