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Preparation and characterization of monoclonal antibodies to the cholera toxin

Abstract

Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.

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Abbreviations

CT:

thermolabile cholera toxin

EIA:

enzyme immunoassay

LT:

thermolabile toxin of E. coli

PBS:

phosphatebuffered saline (pH 7.4)

PBST:

PBS with 0.1% Tween-20

MA:

monoclonal antibodies

SE:

staphyllococ

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Correspondence to T. I. Valyakina.

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Original Russian Text © E.E. Petrova, R.L. Komaleva, O.E. Lakhtina, L.V. Samokhvalova, N. A. Kalinina, N. S. Shoshina, A. Yu. Rubina, M. A. Filippova, Yu.V. Vertiev, T. I. Valyakina, Ye.V. Grishin, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 3, pp. 357–367.

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Petrova, E.E., Komaleva, R.L., Lakhtina, O.E. et al. Preparation and characterization of monoclonal antibodies to the cholera toxin. Russ J Bioorg Chem 35, 326–334 (2009). https://doi.org/10.1134/S1068162009030078

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  • DOI: https://doi.org/10.1134/S1068162009030078

Key words

  • enzyme immunoassay
  • sandwich assay
  • biological microchip
  • B subunit of cholera toxin
  • cholera toxin
  • monoclonal antibodies