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Nanocomplexes of recombinant proteins and polysialic acid: Preparation, characteristics, and biological activity

Russian Journal of Bioorganic Chemistry volume 35, pages 320–325 (2009)Cite this article

Abstract

A preparation of nanocomplexes containing recombinant proteins (interferons α2b and β1b, insulin, and human granulocyte colony stimulating factor) and natural polysialic acid (PSA) has been described. The incorporation of protein into the complex changes its electrophoretic mobility. Atomic force microscopy reveals the average size of 23-kD insulin complexes with PSA of 10–20 nm and demonstrates that more than 60% of glycopolymer molecules carry a single protein molecule. Experiments with cultured cells show that cytokines bound to polysialic acid retain their ability to regulate cell proliferation. Insulin bound to PSA has a prolonged hypoglycemic effect in vivo.

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Abbreviations

G-CSF:

human granulocyte colonysfimulating factor

IFNα2b:

interferon α2β

IFNβ1b:

interferon β1b

HOPG:

high-oriented pyrolyfic graphite

PSA:

polysialie acid

PEG:

polyethylene glycol

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, V-437, Moscow, 117997, Russia

    V. V. Bezuglov, N. M. Gretskaya, D. V. Klinov, M. Yu. Bobrov, E. D. Shibanova, M. G. Akimov, E. V. Fomina-Ageeva, G. N. Zinchenko, D. I. Bairamashvili & A. I. Miroshnikov

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  1. V. V. Bezuglov
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  2. N. M. Gretskaya
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  3. D. V. Klinov
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  4. M. Yu. Bobrov
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  5. E. D. Shibanova
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  6. M. G. Akimov
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  7. E. V. Fomina-Ageeva
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  8. G. N. Zinchenko
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  9. D. I. Bairamashvili
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  10. A. I. Miroshnikov
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Correspondence to V. V. Bezuglov.

Additional information

Original Russian Text © V.V. Bezuglov, N.M. Gretskaya, D.V. Klinov, M.Yu. Bobrov, E.D. Shibanova, M.G. Akimov, E.V. Fomina-Ageeva, G.N. Zinchenko, D.I. Bairamashvili, A.I. Miroshnikov, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 3, pp. 350–356.

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Bezuglov, V.V., Gretskaya, N.M., Klinov, D.V. et al. Nanocomplexes of recombinant proteins and polysialic acid: Preparation, characteristics, and biological activity. Russ J Bioorg Chem 35, 320–325 (2009). https://doi.org/10.1134/S1068162009030066

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  • DOI: https://doi.org/10.1134/S1068162009030066

Key words

  • recombinant human insulin
  • interferons
  • granulocyte colony stimulating factor
  • polysialic acid
  • nanocomplexes