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Isolation of the lipid-transporting protein Ns-LTP1 from seeds of the garden fennel flower (Nigella sativa)

Abstract

A novel lipid-transporting protein (Ns-LTP1) has been isolated from seeds of the garden fennel flower Nigella sativa. The molecular mass, N-terminal amino acid sequence, and amino acid composition of the protein have been determined. Ns-LTP1 has a molecular mass of 9602 Da and contains eight cysteine residues which form four disulfide bridges. The protein is capable of suppressing the development of some phytopathogenic fungi and oomycetes.

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Abbreviations

AMP:

antimicrobial peptides

LTP:

lipid-transporting proteins

References

  1. Kader, J.-C., Julienne, M., and Vergnolle, C., Eur. J. Biochem., 1984, vol. 139, pp. 411–416.

    PubMed  Article  CAS  Google Scholar 

  2. Bouillon, P., Drischel, C., Vergnolle, C., Duranton, H., and Kader, J.-C., Eur. J. Biochem., 1987, vol. 166, pp. 387–391.

    PubMed  Article  CAS  Google Scholar 

  3. Takishima, K., Watanabe, S., Yamada, M., Suga, T., and Mamiya, G., Eur. J. Biochem., 1988, vol. 177, pp. 241–249.

    PubMed  Article  CAS  Google Scholar 

  4. Desormeaux, A., Blochet, J., Pezolet, M., and Marion, D., Biochim. Biophys. Acta, 1992, vol. 1121, pp. 137–152.

    PubMed  CAS  Google Scholar 

  5. Terras, F.R.G., Goderis, I.J., van Leuven, F., Vanderleyden, J., Cammue, B.P.A., and Broekaert, W.F., Plant Physiol., 1992, vol. 100, pp. 1055–1058.

    PubMed  Article  CAS  Google Scholar 

  6. Cammue, B.P.A., De Bolle, M.F.C., and Terras, F.R.G., Plant Physiol., 1995, vol. 109, pp. 445–455.

    PubMed  Article  CAS  Google Scholar 

  7. Nielsen, K.K., Nielsen, J.E., Madrid, S.M., and Mikkelsen, J.D., Plant. Mol. Biol., 1996, vol. 31, pp. 539–552.

    PubMed  Article  CAS  Google Scholar 

  8. del Carmen, Ramirez-Medeles, M., Aguilar, M.B., Miguel, R.N., Bolanos-Garcia, V.M., Garcia-Hernandez, E., and Soriano-Garcia, M., Arch. Biochem. Biophys., 2003, vol. 415, pp. 24–33.

    Article  Google Scholar 

  9. Segura, A., Moreno, M., and Garcia-Olmedo, F., FEBS J., 1993, vol. 332, pp. 243–246.

    Article  CAS  Google Scholar 

  10. Gomar, J., Sodano, P., Silverstein, K.A.T., and Moskal, W.A., Protein, 1998, vol. 31, pp. 160–171.

    Article  CAS  Google Scholar 

  11. Terras, F.R.G., Goderis, I.J., and van Leuven, F., Plant Physiol., 1992, vol. 100, pp. 1055–1058.

    PubMed  Article  CAS  Google Scholar 

  12. Castro, M.S. and Fontes, W., Protein and Peptides Lett., 2005, vol. 12, pp. 11–16.

    Article  Google Scholar 

  13. Kader, J.-C., J. Biol. Chem., 1988, vol. 263, pp. 16849–16855.

    Google Scholar 

  14. Castro, M.S. and Fontes, W., Protein and Peptides Lett., 2005, vol. 12, pp. 11–16.

    Article  Google Scholar 

  15. Hughes, M.A., Heitz, T., and Legrand, M., Plant Cells and Environment, 1992, vol. 15, pp. 861–865.

    Article  CAS  Google Scholar 

  16. Torres-Schumann, S., Godoy, J.F., and Pintor-Toro, J.A., Plant Mol. Biol., 1992, vol. 18, pp. 749–757.

    PubMed  Article  CAS  Google Scholar 

  17. Mollina, A. and Garcia-Olmedo, F., Plant J., 1993, vol. 4, pp. 983–991.

    Article  Google Scholar 

  18. Trevino, M.B. and O’Connell, M.A., Plant Physiol., 1998, vol. 116, pp. 1461–1468.

    PubMed  Article  CAS  Google Scholar 

  19. Franky, R.G., Terras, F.R.G., Bruno Ph.A.C., Broekaert W.F, Plant Physiol., 1993, vol. 103, pp. 1311–1319.

    Google Scholar 

  20. Bruno, Ph.A.C., Thevissen, K., and Broekaert, W.F., Plant Physiol., 1995, vol. 109, pp. 445–455.

    Article  Google Scholar 

  21. Molina, A. and Garcia-Olmedo, F., Plant J., 1997, vol. 12, pp. 669–675.

    PubMed  Article  CAS  Google Scholar 

  22. Garcia-Olmedo, F., Molina, A., Segura, A., Moreno, M., et al., Trends Microbiology, 1995, vol. 3, pp. 72–74.

    Article  CAS  Google Scholar 

  23. Muhammad, A.. and Randhawa, Mastoor S., Al-Ghamdi, Pakistan J. Med. Res., 2002, vol. 41, pp. 72–162.

    Google Scholar 

  24. Mohamed Labib Salem, Int. Immunopharmacol., 2005, vol. 5. P. 1749–1770.

    PubMed  Article  Google Scholar 

  25. Selitrennikoff, P.C., Appl. Environ. Microbiol., 2001, vol. 67, pp. 2883–2894.

    PubMed  Article  CAS  Google Scholar 

  26. Kader, J.-C., Annu. Rev. Plant Physiol. Plant Mol. Biol., 1996, vol. 47, pp. 627–654.

    PubMed  Article  CAS  Google Scholar 

  27. Broekaert, W.F., Cammue, B.P.A., De Bolle, M.F.C., Thevissen, K., De Samblanx, G.W., and Osborn, R.W., Crit. Rev. Plant Sci., 1997, vol. 16, pp. 297–323.

    Article  CAS  Google Scholar 

  28. Federico, M.L., Kaeppler, H.F., and Skadsen, R.W., Plant. Mol. Biol., 2005, vol. 57, pp. 35–51

    PubMed  Article  CAS  Google Scholar 

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Correspondence to Yu. I. Oshchepkova.

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Original Russian Text © Yu.I. Oshchepkova, O.N. Veshkurova, E.A. Rogozhin, A.Kh. Musolyamov, A. N. Smirnov, T.I. Odintsova, Ts.A. Egorov, E.V. Grishin, Sh.I. Salikhov, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 3, pp. 344–349.

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Oshchepkova, Y.I., Veshkurova, O.N., Rogozhin, E.A. et al. Isolation of the lipid-transporting protein Ns-LTP1 from seeds of the garden fennel flower (Nigella sativa). Russ J Bioorg Chem 35, 315–319 (2009). https://doi.org/10.1134/S1068162009030054

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  • DOI: https://doi.org/10.1134/S1068162009030054

Key words

  • plants
  • Nigella sativa
  • antimicrobial peptides
  • lipid-transporting protein
  • antimicrobial activity
  • phytophthora infection