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Antigenic polysaccharides of bacteria: 42. Structures of O-polysaccharides from two Shigella dysenteriae type 8 strains

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Abstract

The structure of the O-polysaccharide (O-antigen) from Shigella dysenteriae type 8 bacteria (strain 599) was corrected using modern NMR techniques (structure 1). The revisions concerned the position of the Glc residue (in the main, but not the side chain), the site of its substitution, and the configuration of the O-glycoside linkage of the GlcNAc residue. The S. dysenteriae type 8 bacterium (strain G1221), the second investigated representative, was found to produce another structural variant of the O-polysaccharide. It contains GlcNAc instead of the Glc residue in the main chain (structure 2). This data may lead to approval of division of S. dysenteriae type 8 into two subtypes:

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Abbreviations

Glc:

glucose

GlcA:

glucuronic acid

GalNAc:

N-acetyl galactosamine

HMBC:

Heteronuclear Multiple Bond Correlation

HSQC:

Heteronuclear Single Quantum Correlation

LPS:

lipopolysaccharide

NAc:

N-acetyl group

OPS:

O-specific polysaccharide chains

ROESY:

nuclear Overhauser Effect Spectroscopy in the Rotating frame

SSCC:

spin-spin coupling constant

TOCSY:

Total Correlation Spectroscopy

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, Moscow, 119991, Russia

    A. V. Perepelov, S. N. Senchenkova, A. S. Shashkov & Yu. A. Knirel

  2. TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa st., TEDA, Tian-jin, 300457, China

    B. Lu, L. Feng & L. Wang

  3. Tian-jin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa str., TEDA, Tian-jin, 300457, China

    B. Lu, L. Feng & L. Wang

Authors
  1. A. V. Perepelov
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  2. S. N. Senchenkova
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  3. A. S. Shashkov
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  4. Yu. A. Knirel
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  5. B. Lu
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  6. L. Feng
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  7. L. Wang
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Corresponding author

Correspondence to A. V. Perepelov.

Additional information

Original Russian Text © A.V. Perepelov, S.N. Senchenkova, A.S. Shashkov, Yu.A. Knirel, B. Lu, L. Feng, and L. Wang, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 6, pp. 808–812.

For communication 41, see [1].

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Perepelov, A.V., Senchenkova, S.N., Shashkov, A.S. et al. Antigenic polysaccharides of bacteria: 42. Structures of O-polysaccharides from two Shigella dysenteriae type 8 strains. Russ J Bioorg Chem 34, 725–729 (2008). https://doi.org/10.1134/S1068162008060125

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  • DOI: https://doi.org/10.1134/S1068162008060125

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