Abstract
A test system for the diagnostics and identification of seven toxigenic fungi causing Fusarium head blight (Fusarium graminearum, F. culmorum, F. poae, F. sporotrichioides, F. langsethiae, F. avenaceum, and F. tricinctum) was developed using PCR. The identification of pathogen is based on the specific amplification of a DNA fragment of the gene of translation elongation factor 1 alpha (tef-1α) and subsequent detection of the results by the fluorescent amplification-based specific hybridization method. The system was tested on 38 isolates of different fungi of the genus Fusarium.
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Abbreviations
- FLASH:
-
fluorescent amplification-based specific hybridization
- PCR:
-
polymerase chain reaction
- IC:
-
internal control
- tef-1α:
-
the gene of the translation elongation factor
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Original Russian Text © D.Yu. Ryazantsev, S.L. Abramova, S.V. Evstratova, T.Yu. Gagkaeva, S.K. Zavriev, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 6, pp. 799–807.
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Ryazantsev, D.Y., Abramova, S.L., Evstratova, S.V. et al. FLASH-PCR diagnostics of toxigenic fungi of the genus Fusarium . Russ J Bioorg Chem 34, 716–724 (2008). https://doi.org/10.1134/S1068162008060113
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DOI: https://doi.org/10.1134/S1068162008060113