Abstract
It has recently been shown that the influenza virus can specifically bind the residue of a nonsialylated sulfated oligosaccharide Gal(6SO3H)β1-4GlcNAcβ (6’SLacNAc). To identify by photoaffinity labeling the virion component that binds 6’SLacNAc, we synthesized a carbohydrate probe containing a 125I labeled diazocyclopentadien-2-yl carbonyl group as an aglycone. According to the electrophoretic data, the labeled areas corresponded to a large hemagglutinin subunit, a nucleocapsid protein, and neuraminidase (NA). Probing in the presence of an excess of 6’SLacNAcβ-OCH2CH2NHAc glycoside resulted in redistribution of the labeling intensity, with the maximum inhibition being observed for NA. The data obtained indicate that NA is a viral 6’SLacNAc-binding protein.
Abbreviations
- Dcp:
-
diazocyclopentadien-2-yl carbonyl
- HA and HA1 :
-
hemagglutinin and its large subunit, respectively
- NA:
-
neuraminidase
- NP:
-
nucleocapside protein
- Np:
-
p-nitrophenyl
- PNGase:
-
glycopeptide N-glycosidase
- Py:
-
pyridine
- Sia:
-
sialic acid
- 6’SiaLacNAc:
-
Gal(6Sia)β1-4GlcNAcβ
- 6’SLacNac:
-
Gal(6SO3H)β1-4GlcNAcβ
- sp:
-
(CH2)2 spacer
- TEA:
-
triethylamine
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Original Russian Text © E.V. Parfinovich, L.V. Mochalova, Yul.G. Molotkovsky, N.V. Bovin, E.L. Vodovozova, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 5, pp. 716–720.
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Parfinovich, E.V., Mochalova, L.V., Molotkovsky, Y.G. et al. Identification of a new carbohydrate-binding site of influenza virus. Russ J Bioorg Chem 34, 642–646 (2008). https://doi.org/10.1134/S1068162008050154
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DOI: https://doi.org/10.1134/S1068162008050154