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Identification of a new carbohydrate-binding site of influenza virus

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Abstract

It has recently been shown that the influenza virus can specifically bind the residue of a nonsialylated sulfated oligosaccharide Gal(6SO3H)β1-4GlcNAcβ (6’SLacNAc). To identify by photoaffinity labeling the virion component that binds 6’SLacNAc, we synthesized a carbohydrate probe containing a 125I labeled diazocyclopentadien-2-yl carbonyl group as an aglycone. According to the electrophoretic data, the labeled areas corresponded to a large hemagglutinin subunit, a nucleocapsid protein, and neuraminidase (NA). Probing in the presence of an excess of 6’SLacNAcβ-OCH2CH2NHAc glycoside resulted in redistribution of the labeling intensity, with the maximum inhibition being observed for NA. The data obtained indicate that NA is a viral 6’SLacNAc-binding protein.

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Abbreviations

Dcp:

diazocyclopentadien-2-yl carbonyl

HA and HA1 :

hemagglutinin and its large subunit, respectively

NA:

neuraminidase

NP:

nucleocapside protein

Np:

p-nitrophenyl

PNGase:

glycopeptide N-glycosidase

Py:

pyridine

Sia:

sialic acid

6’SiaLacNAc:

Gal(6Sia)β1-4GlcNAcβ

6’SLacNac:

Gal(6SO3H)β1-4GlcNAcβ

sp:

(CH2)2 spacer

TEA:

triethylamine

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997, Russia

    E. V. Parfinovich, Yul. G. Molotkovsky, N. V. Bovin & E. L. Vodovozova

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 119991, Russia

    L. V. Mochalova

Authors
  1. E. V. Parfinovich
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  2. L. V. Mochalova
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  3. Yul. G. Molotkovsky
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  4. N. V. Bovin
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  5. E. L. Vodovozova
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Correspondence to E. V. Parfinovich.

Additional information

Original Russian Text © E.V. Parfinovich, L.V. Mochalova, Yul.G. Molotkovsky, N.V. Bovin, E.L. Vodovozova, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 5, pp. 716–720.

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Parfinovich, E.V., Mochalova, L.V., Molotkovsky, Y.G. et al. Identification of a new carbohydrate-binding site of influenza virus. Russ J Bioorg Chem 34, 642–646 (2008). https://doi.org/10.1134/S1068162008050154

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  • DOI: https://doi.org/10.1134/S1068162008050154

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