Abstract
The response of the components of a protease-inhibitor system of legume and cereal crops to stress factors was studied. It was found that salinization, heavy metal ions, and phytopathogenic flora inhibit the activity of neutral, acidic, and alkaline proteases at early stages of seed germination, the degree of the inhibition of the endoprotease activity being dependent on the index of tolerance of legume and cereal crops. It was shown that, in response to unfavorable conditions, accumulation of trypsin inhibitors occurs, which is accompanied by the appearance of new protein components, as indicated by electrophoresis. The results confirm the presumption that serine protease inhibitors are involved in the response of plants to stress factors.
Similar content being viewed by others
Abbreviations
- TI:
-
tolerance index
- BAPase:
-
N α-benzoyl-DL-arginine p-nitroanilide hydrolase
References
Antonov, V.K., Khimiya proteoliza (Chemistry of Proteolysis), Moscow: Nauka, 1991.
Mosolov, V.V. and Valueva, T.A., Rastitel’nye belkovye ingibitory proteoliticheskikh fermentov (Plant Protein Inhibitors of Proteolytic Enzymes), Kretovich, V.L., Ed., Moscow: Bakh Institute of Biochemisry RAN, 1993.
Veremeenko, K.N., Fermenty proteoliza i ikh ingibitory v meditsinskoi praktike (Proteolysis Enzymes and Their Inhibitors in Medical Practice), Kiev: Zdorov’ya, 1971.
Zhu, J.K. and Hasegawa, P.M., and Bressan, R.A., Crit. Rev. Plant Sci., 1997, vol. 16, pp. 253–277.
Veselova, T.V., Veselovskii, V.A., and Chernavskii, D.S., Stress u rastenii (biofizicheskii podkhod) (Stress in Plants: A Biophysical Approach), Moscow: Mosk. Gos. Univ., 1993.
Leah, R. and Mundy, J., Plant. Mol. Biol., 1989, vol. 12, pp. 673–682.
Pena-Cortes, H., Sanchez-Serrano, J.J., Mertens, R., Vilmitzer, L., and Prat, S., Proc. Natl. Acad. Sci. USA, 1989, vol. 86, pp. 9851–9855.
Seregin, I.V. and Ivanov, V.B., Fiziol. Rastenii, 2001, vol. 48, pp. 606–630.
Dunaevskii, Ya.E., Tsybina, T.A., Belyakova, G.N., Domash, V.I., Sharpio, T.P., Zabreiko, S.A., and Belozerskii, M.A., Prikl. Biokhim. Mikrobiol., 2005, vol. 41, pp. 392–396.
Artemuk, E.G., Domash, V.I., and Shurkhai, S.F., Vestnik Brestskogo Un-Ta. Seriya Biol. Nauk, 2004, no. 1 (38), pp. 63–67.
Chase, T. and Shaw, E., Biochem. Biophys. Res. Commun., 1967, vol. 29, pp. 508–514.
Anson, M.L., Abstr. Gen. Physiol., 1938, vol. 22, pp. 79–82.
Erlanger, B.F., Kokowsky, N., and Cohen, W., Arch. Biochem. Biophys., 1961, vol. 95, pp. 271–278.
Gofman, Yu.Ya. and Vaisblai, I.M., Prikl. Biokhim. Mikrobiol., 1975, vol. 11, pp. 777–787.
Aniol, A., Triticale: Today and Tomorrow, Dordrecht, the Netherlands: Kluwer Academic, 1966, pp. 461–465.
Zelinskii, V.G., Nauchno-Tekhn. Byull. Vses. Selekts.-Genet. In-Ta, 1986, no. 1 (59), pp. 37–41.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.I. Domash, T.P. Sharpio, S.A. Zabreiko, T.F. Sosnovskaya, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 3, pp. 353–367.
Rights and permissions
About this article
Cite this article
Domash, V.I., Sharpio, T.P., Zabreiko, S.A. et al. Proteolytic enzymes and trypsin inhibitors of higher plants under stress conditions. Russ J Bioorg Chem 34, 318–322 (2008). https://doi.org/10.1134/S1068162008030114
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1068162008030114