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New indodicarbocyanine dyes for the biological microchip technology

Abstract

New indodicarbocyanine dyes with the carboxybutyl group in position-3 of the indolenine fragment bearing methyl and sulfonic groups in positions 5 and 7 of the cycle were synthesized in order to find the most effective fluorescent labels for the biological microchip technology. The position of absorption and fluorescence maxima, the total charge of the dye molecule, and water solubility depend on the location and the total amount of methyl and sulfonic groups. The spectral characteristics of the dyes synthesized were determined. The relative fluorescence efficiencies of the dyes at equal concentrations were measured at excitation wavelengths of 635 and 655 nm and emission wavelengths of 670 and 690 nm, respectively.

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References

  1. Ranasighe, R.T. and Brown, T., Chem. Communs., 2005, pp. 5487–5502.

  2. Ivanov, I.B., Ershov, G.M., Barskii, V.E., and Mirzabekov, A.D., Mol. Biol. (Moscow), 1997, vol. 31, pp. 159–167.

    CAS  Google Scholar 

  3. Harshman, K. and Martinez, A.C., European Review, 2002, vol. 10, pp. 389–408.

    Article  Google Scholar 

  4. Gerhold, D., Rushmore, T., and Caskey, C.T., Trends in Biochemical Sciences, 1999, vol. 24, pp. 168–173.

    CAS  Article  PubMed  Google Scholar 

  5. Mader, O., Reiner, K., Egelhaaf, H.-J., Fisher, R., and Brock, R., Bioconjugate Chem., 2004, vol. 15, pp. 70–78.

    CAS  Article  Google Scholar 

  6. Mank, A.J.G., van der Laan, H.T.C., Gooijer, C., Brinkman, U.A., and Velthorst, N.H., Anal. Chem., 1995, vol. 67, pp. 1742–1748.

    CAS  Article  Google Scholar 

  7. Mujumdar, R.B., Ernst, L.A., Mujumdar, S.R., Lewis, C.J., and Waggoner, A.S., Bioconjugate Chem., 1993, vol. 4, pp. 105–111.

    CAS  Article  Google Scholar 

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Correspondence to A. V. Chudinov.

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Original Russian Text © V.E. Kuznetsova, V.A. Vasiliskov, O.V. Antonova, V.M. Mikhailovich, A.S. Zasedatelev, A.V. Chudinov, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 1, pp. 141–144.

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Kuznetsova, V.E., Vasiliskov, V.A., Antonova, O.V. et al. New indodicarbocyanine dyes for the biological microchip technology. Russ J Bioorg Chem 34, 130–133 (2008). https://doi.org/10.1134/S1068162008010184

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  • DOI: https://doi.org/10.1134/S1068162008010184

Key words

  • biological microchips
  • fluorescence
  • hybridization analysis
  • indodicarbocyanine dyes