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Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis


The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity.

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human serum albumin


alcohol oxidase


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Correspondence to T. V. Bobik.

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Original Russian Text © T.V. Bobik, I.I. Vorob’ev, N.A. Ponomarenko, A.G. Gabibov, A.I. Miroshnikov, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 1, pp. 56–62.

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Bobik, T.V., Vorob’ev, I.I., Ponomarenko, N.A. et al. Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis. Russ J Bioorg Chem 34, 49–55 (2008).

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Key words

  • expression
  • human serum albumin
  • Pichia pastoris