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Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis

Abstract

The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity.

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Abbreviations

HSA:

human serum albumin

AOX:

alcohol oxidase

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Correspondence to T. V. Bobik.

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Original Russian Text © T.V. Bobik, I.I. Vorob’ev, N.A. Ponomarenko, A.G. Gabibov, A.I. Miroshnikov, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 1, pp. 56–62.

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Bobik, T.V., Vorob’ev, I.I., Ponomarenko, N.A. et al. Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis. Russ J Bioorg Chem 34, 49–55 (2008). https://doi.org/10.1134/S1068162008010068

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  • DOI: https://doi.org/10.1134/S1068162008010068

Key words

  • expression
  • human serum albumin
  • Pichia pastoris