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Interaction of the synthetic immunomodulatory dipeptide bestim with murine macrophages and thymocytes

Russian Journal of Bioorganic Chemistry volume 34, pages 37–42 (2008)Cite this article

Abstract

The tritium-labeled dipeptide bestim (γ-D-Glu-L-Trp) with a specific activity of 45 Ci/mmol was obtained by high-temperature solid-state catalytic isotope exchange. It was found that [3H]bestim binds with a high affinity to murine peritoneal macrophages (K d 2.1 ± 0.1 nM) and thymocytes (K d 3.1 ± 0.2 nM), as well as with plasma membranes isolated from these cells (K d 18.6 ± 0.2 and 16.7 ± 0.3 nM, respectively). The specific binding of [3H]bestim to macrophages and thymocytes was inhibited by the unlabeled dipeptide thymogen (L-Glu-L-Trp) (K i 0.9 ± 0.1 and 1.1 ± 0.1 nM, respectively). After treatment with trypsin, macrophages and thymocytes lost the ability to bind [3H]bestim. Bestim in the concentration range of 10−10 to 10−6 M reduced the adenylate cyclase activity in the membranes of murine macrophages and thymocytes.

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Abbreviations

ACTH:

adrenocorticotropic hormone

PAM:

phenacylamidomethyl

γ-D-Glu:

the glutamic acid residue that forms the peptide bond through the γ-carboxyl group

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Authors and Affiliations

  1. State Research Center, Institute of Extrapure Biopreparations, FMBA of the Russian Federation, St. Petersburg, 197110, Russia

    A. A. Kolobov & N. I. Kolodkin

  2. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, Moscow, 123182, Russia

    Yu. A. Zolotarev

  3. SciClone Pharmaceuticals Inc., 901, Mariner’s Island Blvd., San Mateo, CA, 9404-1593, USA

    C. Tuthill

  4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow oblast, 142290, Russia

    E. V. Navolotskaya

Authors
  1. A. A. Kolobov
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  2. N. I. Kolodkin
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  3. Yu. A. Zolotarev
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  4. C. Tuthill
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  5. E. V. Navolotskaya
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Corresponding author

Correspondence to E. V. Navolotskaya.

Additional information

Original Russian Text © A.A. Kolobov, N.I. Kolodkin, Yu.A. Zolotarev, C. Tuthill, E.V. Navolotskaya, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 1, pp. 43–49.

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Kolobov, A.A., Kolodkin, N.I., Zolotarev, Y.A. et al. Interaction of the synthetic immunomodulatory dipeptide bestim with murine macrophages and thymocytes. Russ J Bioorg Chem 34, 37–42 (2008). https://doi.org/10.1134/S1068162008010044

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  • DOI: https://doi.org/10.1134/S1068162008010044

Key words

  • adenylate cyclase
  • immune system
  • peptides
  • receptors