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Isolation and elucidation of structure of peptides from oat (Avena sativa) seedlings

Abstract

The level of proteolytic activity in tissues of oat seedlings was characterized under acidic conditions, and the number and content of the main components in low-molecular-mass fractions of the extract were determined. The structures of the majority of predominant peptide components isolated from the extract were studied. The use of a database of protein structures helped suggest possible structures of protein precursors of the peptides isolated. Detailed information on a plant peptidome was obtained for the first time.

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Abbreviations

EDTA:

ethylenediaminetetraacetatic acid disodium salt

PMSF:

phenylmethylsulfonyl fluoride

rpHPLC:

reversed phase high performance liquid chromatography

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Correspondence to O. G. Gara.

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Original Russian Text © O.G. Gara, O.N. Yatskin, V.I. Shvets, A.A. Karelin, V.T. Ivanov, 2006, published in Bioorganicheskaya Khimiya, 2006, Vol. 32, No. 2, pp. 211–220.

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Gara, O.G., Yatskin, O.N., Shvets, V.I. et al. Isolation and elucidation of structure of peptides from oat (Avena sativa) seedlings. Russ J Bioorg Chem 32, 191–200 (2006). https://doi.org/10.1134/S1068162006020129

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  • DOI: https://doi.org/10.1134/S1068162006020129

Key words

  • peptides of plant tissues
  • isolation
  • identification
  • protein precursor