Skip to main content
Log in

Isolation and elucidation of structure of peptides from oat (Avena sativa) seedlings

Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

The level of proteolytic activity in tissues of oat seedlings was characterized under acidic conditions, and the number and content of the main components in low-molecular-mass fractions of the extract were determined. The structures of the majority of predominant peptide components isolated from the extract were studied. The use of a database of protein structures helped suggest possible structures of protein precursors of the peptides isolated. Detailed information on a plant peptidome was obtained for the first time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EDTA:

ethylenediaminetetraacetatic acid disodium salt

PMSF:

phenylmethylsulfonyl fluoride

rpHPLC:

reversed phase high performance liquid chromatography

References

  1. Ivanov, V.T., Karelin, A.A., Mikhaleva, I.I., Vas’kovskii, B.V., Sviryaev, V.I., and Nazimov, I.V., Bioorg. Khim., 1992, vol. 18, pp. 1271–1311.

    CAS  Google Scholar 

  2. Karelin, A.A., Philippova, M.M., Karelina, E.V., Strizhkov, B.N., Grishina, G.A., Nazimov, I.V., and Ivanov, V.T., J. Peptide Sci., 1998, vol. 4, pp. 211–225.

    Article  CAS  Google Scholar 

  3. Ivanov, V.T., Karelin, A.A., Philippova, M.M., Nazimov, I.V., and Pletnev, V.Z., Biopolymers, Peptide Science, 1997, vol. 43, pp. 171–188.

    CAS  Google Scholar 

  4. Blishchenko, E.Yu., Mernenko, O.A., Mirkina, I.I., Satpaev, D.K., Ivanov, V.S., Tchirkin, L.D., Ostrovsky, A.G., Karelin, A.A., and Ivanov, V.T., Peptides, 1997, vol. 18, pp. 79–85.

    PubMed  CAS  Google Scholar 

  5. Franssen, H. and Bisseling, T., Proc. Natl. Acad. Sci. USA, 2001, vol. 98, pp. 12 855–12 856.

    Article  CAS  Google Scholar 

  6. Matsubayashi, Y., J. Cell Sci., 2003, vol. 116, pp. 3863–3870.

    Article  PubMed  CAS  Google Scholar 

  7. Ivanov, V.T., Karelin, A.A., and Yatskin, O.N., Biopolymers, 2005, vol. 80, pp. 332–346.

    Article  PubMed  CAS  Google Scholar 

  8. Holley, S.R., Yalamanchili, R.D., Moura, D.S., Ryan, C.A., and Stratmann, J.W., Plant Physiol., 2003, vol. 132, pp. 1728–1738.

    Article  PubMed  CAS  Google Scholar 

  9. Ryan, C.A., Biochim. Biophys. Acta, 2000, vol. 1477, pp. 112–121.

    PubMed  CAS  Google Scholar 

  10. Constabel, C.P., Yip, L., and Ryan, C.A., Plant. Mol. Biol., 1996, vol. 36, pp. 55–62.

    Google Scholar 

  11. Matsubayashi, Y., Takagi, L., and Sakagami, Y., Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 13 357–13 362.

    Article  CAS  Google Scholar 

  12. Yang, H., Matsubayashi, Y., Hanai, H., and Sakagami, Y., Plant Cell Physiol., 2000, vol. 41, pp. 825–830.

    PubMed  CAS  Google Scholar 

  13. Hanai, H., Matsuno, T., Yamamoto, M., Matsubayashi, Y., Kobayashi, T., Kamada, H., and Sakagami, Y., Plant Cell Physiol., 2000, vol. 41, pp. 27–32.

    PubMed  CAS  Google Scholar 

  14. Clark, S.E., Running, M.P., and Meyerowitz, E.M., Development, 1993, vol. 119, pp. 397–418.

    PubMed  CAS  Google Scholar 

  15. Fletcher, J.C., Brand, U., Running, M.P., Simon, R., and Meyerowitz, E.M., Science, 1999, vol. 283, pp. 1911–1914.

    Article  PubMed  CAS  Google Scholar 

  16. Karelin, A.A., Philippova, M.M., Yatskin, O.N., Kalinina, O.A., Nazimov, I.V., Blishchenko, E.Yu., and Ivanov, V.T., J. Peptide Sci., 2000, vol. 6, pp. 345–354.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to O. G. Gara.

Additional information

Original Russian Text © O.G. Gara, O.N. Yatskin, V.I. Shvets, A.A. Karelin, V.T. Ivanov, 2006, published in Bioorganicheskaya Khimiya, 2006, Vol. 32, No. 2, pp. 211–220.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gara, O.G., Yatskin, O.N., Shvets, V.I. et al. Isolation and elucidation of structure of peptides from oat (Avena sativa) seedlings. Russ J Bioorg Chem 32, 191–200 (2006). https://doi.org/10.1134/S1068162006020129

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162006020129

Key words

Navigation