Abstract
The possibility of a correlation between the membrane properties of the delta sleep-inducing peptide (DSIP) and its analogues and their biological activity in vivo was examined by a comparative study of the membrane effects of these peptides. The peptides exhibiting biological activity in vivo were shown to cause a statistically reliable disordering of lipids in thrombocyte plasma membranes similar to the effect of DSIP. The membrane effect of the D-Val2-, D-Tyr2-, and Tyr1, Pro2 analogues of DSIP had the same bimodal dose dependence characteristic of natural DSIP. Only a slight nonspecific lipid disordering was registered for Trp-Asp-Ala-Ser-Gly-Glu, a biologically inactive hexapeptide analogue. These results indicate a correlation between the biological activity of the peptides during in vivo tests and their membrane properties in vitro. The structure-function relationship was studied within the group of DSIP analogues examined in vitro. The DSIP modeling effect, especially pronounced under the action of stress factors, was suggested to be directly associated with the ability of DSIP to change the dynamic structure of biological membranes.
Abbreviations
- DSIP:
-
delta sleep-inducing peptide
- ID-1:
-
the Trp-D-Val-Gly-Gly-Asp-Ala-Ser-Gly-Glu analogue of DSIP
- ID-2:
-
the Trp-D-Tyr-Gly-Gly-Asp-Ala-Ser-Gly-Glu analogue of DSIP
- ID-5:
-
the Tyr-Pro-Gly-Gly-Asp-Ala-Ser-Gly-Glu analogue of DSIP
- ID-31:
-
the H-Trp-Asp-Ala-Ser-Gly-Glu-OH analogue of DSIP [5]
References
Monnier, M., Dudler, L., Gaechter, R., and Schoenenberger, G.A., Neurosci. Lett., 1977, vol. 6, pp. 9–13.
Graf, M.V. and Kastin, A.J., Peptides, 1986, vol. 7, pp. 1165–1187.
Pollard, B.J. and Pomfrett, C.J.D., Eur. J. Anaesthesiol., 2001, vol. 18, pp. 419–422.
Rikhireva, G.T., Golubev, I.N., Kopylovskii, S.A., Prudchenko, I.A., and Mikhaleva, I.I., Rus. J. Bioorg. Khim., 1999, vol. 25, pp. 292–297.
Rikhireva, G.T., Golubev, I.N., Prudchenko, I.A., and Mikhaleva, I.I., Biol. Membr., 2003, vol. 20, pp. 409–418.
Kastin, A.J., Banks, W.A., Castellanos, P.F., Nissen, C., and Coy, D.H., Pharmacol. Biochem. Behav., 1982, vol. 17, pp. 1187–1191.
Raeissi, S. and Audus, K.L., J. Pharmacol., 1989, vol. 41, pp. 848–852.
Nabiev, I.R., Efremov, E.S., Sargsyan, A.S., Mikhaleva, I.I., and Ivanov, V.T., Bioorg. Khim., 1982, vol. 8, pp. 900–904.
Mikhaleva, I.I., Sargsyan, A.C., Balashova, T.A., and Ivanov, V.T., in Chemistry of Peptides and Proteins, Voelter, W., Wunsch, E., Ovchinnikov, J., and Ivanov, V., Eds., Berlin: Walter de Gruyter, 1982, pp. 289–297.
Gray, R.A., Vander Velde, D.G., Burke, C.J., Manning, M.C., Middaugh, C.R., and Borchardt, R.T., Biochemistry, 1994, vol. 33, pp. 1323–1331.
Polverini, E., Casadio, R., Neyroz, P., and Masotti, L., Arch. Biochem. Biophys., 1998, vol. 349, pp. 225–235.
Rikhireva, G.T., Makletsova, M.G., Mendzheritskii, A.M., Vartanyan, L.S., Gurevich, S.M., Lozovskaya, E.L., Kopylovskii, S.A., Rylova, A.V., Prudchenko, I.A., and Mikhaleva, I.I., Izv. Ross. Acad. Nauk, Ser. Biol., 1993, no. 2, pp. 243–256.
Khvatova, E.M., Rubanova, N.A., Prudchenko, I.A., and Mikhaleva, I.I., FEBS Lett., 1995, vol. 368, pp. 367–369.
Shustanova, T.A., Bondarenko, T.I., Milyutina, N.P., and Mikhaleva, I.I., Biokhimiya (Moscow), 2001, vol. 66, pp. 780–789.
Prudchenko, I.A., Stashevskaya, L.V., Mikhaleva, I.I., Ivanov, V.T., Shandra, A.A., Godlevskii, L.S., and Mazarati, A.M., Bioorg. Khim., 1993, vol. 19, pp. 43–55.
Mikhaleva, I.I., Prudchenko, I.A., Mendzheritskii, A.M., and Vilkov, G.A., RF Patent 2 115 660 (1998).
Urban, V.P., Voitenkov, B.O., Shirobokova, M.M., Smirnov, A.V., Tishko, I.A., and Valeeva, S.V., Del’tatsin (“Zoolan”): Use in Veterinary Practice, Methodological Prescriptions, St. Petersburg: State St. Petersburg Veterinary Academy, 2001, pp. 12–15.
Prudchenko, I.A., Stashevskaya, L.V., Shepel’, E.N., Mikhaleva, I.I., Ivanov, V.T., Shmal’ko, Yu.P., Chalyi, A.P., Umanskii, V.Yu., and Grinzhevskaya, S.N., Bioorg. Khim., 1993, vol. 19, pp. 1177–1190.
Popovich, I.G., Voitenkov, B.O., Anisimov, V.N., Ivanov, V.T., Mikhaleva, I.I., Zabezhinski, M.A., Alimova, I.N., Baturin, D.A., Zavarzina, N.I., Rosenfeld, V., Semenchenko, A.V., and Yashin, A.I., Mech. Ageing Dev., 2003, vol. 124, pp. 721–731.
Tsuda, K. and Nishio, I., Am. J. Hypertens., 2003, vol. 16, pp. 259–261.
Choe, M., Jackson, C., and Pal Yu B., Free Radicals Biology & Medicine, 1995, vol. 18, pp. 944–977.
Zicha, J., Kunes, J., and Devynck, M.A., Am. J. Hypertens, 1999, vol. 12, pp. 315–331.
Gerrard, J.M., Methods Enzymol., 1982, vol. 86, pp. 642–654.
Kuznetsov, A.N., in Metod spinovogo zonda (osnovy i primenenie) (Method of Spin Probes: Fundamentals and Use), Moscow: Nauka, 1976.
Griffith, O.H. and Jost, P.C., in Spin Labeling: Theory and Application, Berliner, L.J., Ed., New York: Academic, 1976, pp. 489–569.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © I.I. Mikhaleva, G.T. Rikhireva, I.A. Prudchenko, I.N. Golubev, 2006, published in Bioorganicheskaya Khimiya, 2006, Vol. 32, No. 2, pp. 176–182.
Rights and permissions
About this article
Cite this article
Mikhaleva, I.I., Rikhireva, G.T., Prudchenko, I.A. et al. Interaction of delta sleep-inducing peptide and its analogues with cellular membranes: A structure-function analysis. Russ J Bioorg Chem 32, 160–165 (2006). https://doi.org/10.1134/S1068162006020087
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1134/S1068162006020087