Skip to main content

AFLP analysis to estimate the genetic contribution of parents to progeny from hybridization between Saxifraga sibirica L. and S. cernua L.

Abstract

It is shown that the method of amplified fragment length polymorphism (AFLP) can be used to estimate the contribution of parent plants to the genome of the progeny from artificial crosses between Saxifraga cernua and S. sibirica. According to Nei’s (1972) genetic distances between plant groups, F1 plants are intermediate between the parent species but closer to S. cernua, probably because its genome size is twice that of S. sibirica. Conversely, B1 plants proved to be closer to S. sibirica, because the hybrid progeny were crossed back to this species.

This is a preview of subscription content, access via your institution.

References

  • Arnold, M.L., Natural Hybridization As Evolution Process, Ann. Rev. Ecol. Syst., 1992, vol. 23, pp. 237–261.

    Article  Google Scholar 

  • Bauert, M.R., Kaelin, M., Baltisberger, M., and Edwards, P.J., No Genetic Variation Detected within Isolated Relict Populations of the Saxifraga cernua in the Alps Using RAPD Markers, Mol. Ecol., 1998, no. 7, pp. 1519–1527.

  • Bronken, P., Circumpolar Chloroplast and Nuclear DNA Variation in Saxifraga cernua, Cand. Sci. Thesis, Oslo, 2001.

  • Cloutier, S., Cappadocia, M., and Landry, B.S., Analysis of RELP Mapping Inaccuracy in Brassica napus L, Theor. Appl. Genet., 1997, no. 95, pp. 83–91.

  • Dymshakova, O.S., Artificial Hybridization between Saxifraga cernua L. and S. sibirica L., Russ. J. Ecol., 2009, no. 6, pp. 465–467.

  • Funamoto, T., Kondo, K., Hong, D., et al., A Chromosome Study of Five Saxifraga Species Collected in Qin Ling Mountains, Shaanxi Province, China, Chromosome Sci., 1998, vol. 2, pp. 145–150.

    Google Scholar 

  • Gobert, V., Hybridization in the Section Mentha (Lamiaceae) Inferred from AFLP Markers, Am. J. Bot., 2002, vol. 89, no. 12, pp. 2017–2023.

    PubMed  Article  CAS  Google Scholar 

  • Gorchakovskii, P.L., Basic Stages in the Formation of Ural Flora and Vegetation in the Cenozoic According to Paleobotanical Data: The Quaternary Period (Anthropogene), in Osnovnye problemy istoricheskoi fitogeografii Urala (Main Problems in the Historical Phytogeography of the Urals), Sverdlovsk, 1969, pp. 20–37.

  • Grant, V., Plant Speciation, New York: Columbia Univ. Press, 1981. Translated under the title Vidoobrazovanie u rastenii, Moscow: Mir, 1984.

    Google Scholar 

  • Huff, D.R., Peacall, R., and Smouse, P.E., RAPD Variation within and among Natural Populations of Outcrossing Buffalograss Buchloe dactiloides (Nutt) Engelm., Theor. Appl. Genet., 1993, vol. 86, pp. 927–934.

    Article  CAS  Google Scholar 

  • Kaplan, Z. and Fehrer, J., Comparison of Natural and Artificial Hybridization in Potamogeton, Presila, 2006, vol. 78, pp. 303–316.

    Google Scholar 

  • Kapralov, M.V., Population Structure of the Saxifraga cernua L.-S. sibirica L. Complex in the Urals and Factors Determining It, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Yekaterinburg, 2004.

  • Kapralov, M.V., Gabrielsen, T.M., Sarapultsev, I.E., et al., Genetic Enrichment of the Arctic Clonal Plant Saxifraga cernua at Its Southern Periphery via the Alpine Sexual Saxifraga sibirica, Mol. Ecol., 2006, vol. 15, pp. 3401–3411.

    PubMed  Article  CAS  Google Scholar 

  • Li, Y.H., Han, Z.H., and Xu, X., Segregation Patterns of AFLP Markers in F1 Hybrids of a Cross Between Tetraploid and Diploid Species in the Genus Malus, Plant Breed., 2004, no. 123, pp. 316–320.

  • Lowe, A.J. and Abbott, R., Routes of Origin of Two Recently Evolved Hybrid Taxa: Senecio vulgaris Var. Hibernicus and York Radiate Groundsel (Asteraceae), Am. J. Bot., 2000, vol. 87, no. 8, pp. 1159–1167.

    PubMed  Article  CAS  Google Scholar 

  • Murín, A., Hàberovà, T., and Žamsran, C., Further Karyological Studies of the Mongolian Flora, Folia Geobot. Phytotaxon, 1984, vol. 19, pp. 29–40.

    Google Scholar 

  • Nei, M., Genetic Distance between Populations, Am. Nat., 1972, vol. 106, pp. 283–292.

    Article  Google Scholar 

  • Pan, J.T., Gornall, R., and Ohba, H., Saxifraga Linnaeus, sp. pl. 1., in Flora of China, Beijing: Sci. Press, 2001, vol. 8, pp. 280–344.

    Google Scholar 

  • Peakall, R. and Smouse, P.E., GenAlEx 6: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research, Mol. Ecol. Notes, 2006, vol. 6, pp. 288–295.

    Article  Google Scholar 

  • Ramsey, J. and Schemske, D.W., Pathway, Mechanisms, and Rates of Polyploid Formation in Flowering Plants, Annu. Rev. Ecol. Syst., 1998, vol. 29, pp. 267–501.

    Article  Google Scholar 

  • Rieseberg, L.H., Homoploid Reticulate Evolution in Helianthus (Asteraceae): Evidence from Ribosomal Genes, Am. J. Bot., 1991, vol. 78, pp. 1218–1237.

    Article  Google Scholar 

  • Svejda, F., Reproductive Capacity of F1 Hybrids from Rosa rugosa and chinensis Cultivars, Euphytica, 1974, vol. 23, pp. 665–669.

    Article  Google Scholar 

  • Tsvelev, N.N., On the Significance of Hybridization Processes in the Evolution of Grasses, in Istoriya flory i rastitel’nosti Evrazii (The History of Eurasian Flora and Vegetation), Leningrad: Nauka, 1972, pp. 5–15.

    Google Scholar 

  • Tsvelev, N.N., Hybridization As a Factor Increasing Biological Diversity, and Genomic Criteria of Genera in Higher Plants, Biologicheskoe raznoobrazie: podkhody k izucheniyu i sokhraneniyu: Mat-ly konf. (Proc. Conf. “Biological Diversity: Approaches to Study and Conservation,”), Bot. Inst. and Zool. Inst. Ross. Akad. Nauk, St. Petersburg, 1992, pp. 193–201.

    Google Scholar 

  • Vos, P., Hogers, R., Bleeker, M., et al., AFLP: A New Technique for DNA Fingerprinting, Nucleic Acids Res., 1995, vol. 23, no. 21, pp. 4407–4414.

    PubMed  Article  CAS  Google Scholar 

  • Zhmylev, P.Yu., Hybridization in Saxifrages and Its Significance for the Evolution of the Genus Saxifraga L. (Saxifragaceae), Byull. Mosk. O-va Ispyt. Prir., Otd. Biol., 2002, vol. 107, no. 6, pp. 19–28.

    Google Scholar 

  • Zhukova, P.G. and Petrovskii, P.P., Karyotaxonomic Analysis of Some Species of the Genus Saxifraga (Saxifragaceae) from Northern Asia, Bot. Zh., 1987, vol. 72, no. 5, pp. 632–640.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to O. S. Dymshakova.

Additional information

Original Russian Text © O.S. Dymshakova, V.L. Semerikov, M. Lascoux, 2012, published in Ekologiya, 2012, No. 5, pp. 319–323.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dymshakova, O.S., Semerikov, V.L. & Lascoux, M. AFLP analysis to estimate the genetic contribution of parents to progeny from hybridization between Saxifraga sibirica L. and S. cernua L.. Russ J Ecol 43, 347–351 (2012). https://doi.org/10.1134/S1067413612050062

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1067413612050062

Keywords

  • Saxifraga cernua
  • S. sibirica
  • AFLP
  • artificial crossing
  • hybridization