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Technetium-99m Labeling of Antineoplaston A10 and Its Bioevaluation as a Potential Tumor Imaging Agent

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Abstract

Antineoplastons (ANPs) are compounds that have antitumor activities. ANPA10 was labeled with technetium-99m using stannous chloride as a reducing agent with the labeling yield of approximately 98% at pH 6. The conditions required to optimize the labeling yield were studied in details. The tumor binding specificity of the [99mTc]Tc-ANPA10 radiotracer was evaluated. The uptake of [99mTc]Tc-ANPA10 in ascitic fluid uptake was 10, 16, and 12.8% ID/g at 30, 60, and 120 min post injection. The preclinical biological evaluation of [99mTc]Tc-ANPA10 complex in solid tumor bearing mice showed a high tumor uptake at 1 h post injection (11.2 ± 0.30 % ID/g). The selective uptake of [99mTc]Tc-ANPA10 in solid tumors and rapid clearance from nontarget organs make it promising for solid tumor imaging, but further clinical trials are required.

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REFERENCES

  1. Aboumanei, M.H., Abdelbary, A.A., Ibrahim, I.T., Tadros, M.I., and El-Kolaly, M.T., Int. J. Pharm., 2018, vol. 545, nos. 1–2, pp. 240–253.

    Article  CAS  Google Scholar 

  2. Aboumanei, M.H., Abdelbary, A.A., Ibrahim, I.T., Tadros, M.I., and El-Kolaly, M.T., Cancer Biother. Radiopharm., 2018, vol. 33, no. 6, pp. 221–232.

    Article  CAS  Google Scholar 

  3. Figg, W.D., Lancet Oncol., 2018, vol. 19, no. 6, pp. 733–734. https://doi.org/10.1016/S1470-2045(18)30338-3

    Article  PubMed  Google Scholar 

  4. Badria, F., Mabed, M., El-Awadi, M., Abou-Zeid, L., Al-Nashar, E., and Hawas, S., Cancer Lett., 2000, vol. 157, no. 1, pp. 57–63.

    Article  CAS  Google Scholar 

  5. Burzynski, S.R., Efficacy of antineoplastons A-10 and AS2-1, in Mayo Clinic Proc.,Amsterdam: Elsevier, 1999.

    Google Scholar 

  6. Sołtysiak-Pawłuczuk, D. and Burzyński, S.R., Cancer Lett., 1995, vol. 88, no. 1, pp. 107–112.

    Article  Google Scholar 

  7. Othman, S.A. et al., ALL AUTHORS IOP Conf. Ser. Mater. Sci. Eng., 2019. Vol. 555, ID 012032.

  8. Binion, A.K., Radiopharmaceuticals: the Application of Technetium-99m and Rhenium Complexes, A Capstone Project Submitted in Partial Fulfillment of the Requirements of the Renée Crown University Honors Program at Syracuse University, Syracuse, 2014.

  9. NIH (National Institutes of Health) Guide for the Care and Use of Laboratory Animals, Washington, DC: National Academy, 1996, 7th ed.

  10. El-Kawy, O., Ibrahim, I., and Farah, K., J. Label. Compd. Radiopharm., 2015, vol. 58, no. 8, pp. 336–341.

    Article  CAS  Google Scholar 

  11. El-Kawy, O. and Talaat, H., J. Label. Compd. Radiopharm., 2016, vol. 59, no. 2, pp. 72–77.

    Article  CAS  Google Scholar 

  12. Hoye, A., J. Chromatogr, 1967, vol. 28, no. 2, pp. 379–384. https://doi.org/10.1016/s0021-9673(01)85979-3

    Article  CAS  PubMed  Google Scholar 

  13. Rajkapoor, B., Jayakar, B., and Murugesh, N., Indian J. Pharmacol., 2004, vol. 36, pp. 38–40.

    Google Scholar 

  14. Abd El-Rahman, A.M., Mohamed, A.A., Mohamed, S.A., and Mostafa, M.A., Pain Med., 2018, vol. 19, no. 3, pp. 561–568.

    Article  Google Scholar 

  15. Motaleb, M. and Sakr, T., J. Label. Compd. Radiopharm., 2011, vol. 54, no. 9, pp. 597–601.

    Article  CAS  Google Scholar 

  16. Spies, H. and Pietzsch, H.J., Stannous Chloride in the Preparation of 99mTc Pharmaceuticals, Technetium-99m Pharmaceuticals, Zolle, I., Ed., Berlin: Springer, 2007. https://doi.org/10.1007/978-3-540-33990-8_3

    Book  Google Scholar 

  17. El-Ghany, E.A., Marzouk, F., Abd El-Azim, S.A., Awwad, M.H., and Barakat, M.A., Egypt. J. Hospital Med., 2007, vol. 29, pp. 647–663.

    Article  CAS  Google Scholar 

  18. Zhang, J., Yu, Q., Huo, J., Pang, Y., Yang, S., He, Y., Tang, T., Yang, C., and Wang, X., J. Radioanal. Nucl. Chem., 2010, vol. 283, no. 2, pp. 481–485.

    Article  CAS  Google Scholar 

  19. Sakr, T., Motaleb, M., and Ibrahim, I., J. Radioanal. Nucl. Chem., 2012, vol. 292, no. 2, pp. 705–710.

    Article  CAS  Google Scholar 

  20. Sakr, T., Essa, B., El-Essawy, F., and El-Mohty, A., Radiochemistry, 2014, vol. 56, no. 1, pp. 76–80.

    Article  CAS  Google Scholar 

  21. De Barros, A.L.B., das Graças Mota, L., de Aguiar Ferreira, C., de Oliveira, M.C., de Góes, A.M., and Cardoso, V.N., Bioorg. Med. Chem. Lett., 2010, vol. 20, no. 21, pp. 6182–6184.

    Article  Google Scholar 

  22. Ding, R., He, Y., Xu, J., Liu, H., Wang, X., Feng, M., Qi, C., Zhang, J., and Peng, C., Med. Chem. Res., 2012, vol. 21, no. 4, pp. 523–530.

    Article  CAS  Google Scholar 

  23. Sakr, T., El-Safoury, D., Awad, G.A., and Motaleb, M., J. Label. Compd. Radiopharm., 2013, vol. 56, no. 8, pp. 392–395.

    Article  CAS  Google Scholar 

  24. Abou-Zeid, L., El-Mowafy, A.M., El-Kerdawy, M.M., Hamza, H., and Abdel-Hamid, M.E., Farmaco, 2001, vol. 56, no. 10, pp. 763–770.

    Article  CAS  Google Scholar 

  25. Mishra, S., Tamta, A.K., Sarikhani, M., et al., ALL AUTHORS Sci. Rep., 2018, vol. 8, p. 5599.

    Google Scholar 

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Correspondence to I. T. Ibrahim.

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Dawood, M., Alani, B.G., Salim, K.S. et al. Technetium-99m Labeling of Antineoplaston A10 and Its Bioevaluation as a Potential Tumor Imaging Agent. Radiochemistry 64, 219–227 (2022). https://doi.org/10.1134/S106636222202014X

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  • DOI: https://doi.org/10.1134/S106636222202014X

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