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Assessment of Ecotoxicity of Silver Particles Different in Size according to Biological Indicators in Haplic Chernozem

  • DEGRADATION, REHABILITATION, AND CONSERVATION OF SOILS
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Abstract

The ecotoxicity of Ag particles of different size has been assessed by microbiological, biochemical, and phytotoxic indicators for the upper layer (0–20 cm) of ordinary chernozem (Haplic Chernozem) in a laboratory model experiment. The effect has been studied of nano- (10 and 100 nm) and microparticles (1000 nm) of Ag at concentrations of 1, 10, and 100 mg/kg on the biological parameters of ordinary chernozem 30 days after contamination: the activity of catalase, dehydrogenases, ferrireductase, urease, peroxidase, polyphenol oxidase, invertase, phosphatase, the total number of bacteria, the abundance of bacteria of Azotobacter genus, the number of germinated seeds and the length of radish roots. It was found that the ecotoxicity of Ag particles depended on their size: in most cases, Ag particles 10 nm in size had a stronger ecotoxic effect on the biological parameters than particles 100 and 1000 nm in size. There were no significant differences in the ecotoxicity of 100 and 1000 nm Ag particles. The difference in the effects of Ag particles of different sizes increased with increasing Ag concentration in the soil: the higher the Ag concentration was in the soil (from 1 to 100 mg/kg), the more pronounced the difference was in ecotoxicity between 10 nm Ag particles and 100 and 1000 nm Ag particles. Phytotoxic indicators were more sensitive to contamination by Ag nanoparticles at all concentrations studied (1, 10 and 100 mg/kg); the total number of bacteria, invertase and phosphatase activity at 10 and 100 mg/kg; the abundance of bacteria of Azotobacter genus and the activity of dehydrogenases at 100 mg/kg. It is advisable to use these indicators in biodiagnostics of the ecotoxicity of Ag nanoparticles.

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Funding

This work was supported by Russian Science Foundation, project no. 22-74-00054 in Southern Federal University (https://rscf.ru/project/22-74-00054/).

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  1. Southern Federal University, Academy of Biology and Biotechnology, 344090, Rostov-on-Don, Russia

    N. I. Tsepina, S. I. Kolesnikov, T. V. Minnikova, A. S. Ruseva, D. A. Trufanov & K. S. Kazeev

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Tsepina, N.I., Kolesnikov, S.I., Minnikova, T.V. et al. Assessment of Ecotoxicity of Silver Particles Different in Size according to Biological Indicators in Haplic Chernozem. Eurasian Soil Sc. 57, 865–874 (2024). https://doi.org/10.1134/S1064229323603645

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