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Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm

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Abstract

The decay of polarized fluorescence in coenzyme NADH in aqueous-methanol solution under two-photon excitation by femtosecond laser pulses within the spectral range of 720–780 nm has been studied. Molecular fluorescence parameters: decay times τ1 and τ2, rotational diffusion time τrot, and pre-exponential factor ratio a2/a1 have been determined from experiment. The results obtained have been compared with those reported recently in NADH in aqueous solution.

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ACKNOWLEDGMENTS

The authors are grateful to the Ioffe Institute for providing the equipment used in the experiments.

Funding

The study was carried out under the support of the Russian Foundation for Basic Research, project no. 18-53-34001 Cuba_t.

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Authors and Affiliations

  1. Ioffe Physical Technical Institute, Russian Academy of Sciences, 194021, St. Petersburg, Russia

    M. E. Sasin, I. A. Gorbunova, N. O. Bezverkhnii, Y. M. Beltukov & O. S. Vasyutinskii

  2. Universidad de La Habana, La Habana, Cuba

    J. Rubayo-Soneira

Authors
  1. M. E. Sasin
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  2. I. A. Gorbunova
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  3. N. O. Bezverkhnii
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  4. Y. M. Beltukov
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  5. O. S. Vasyutinskii
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  6. J. Rubayo-Soneira
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Correspondence to M. E. Sasin.

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Sasin, M.E., Gorbunova, I.A., Bezverkhnii, N.O. et al. Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm. Tech. Phys. Lett. 45, 672–674 (2019). https://doi.org/10.1134/S1063785019070101

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  • DOI: https://doi.org/10.1134/S1063785019070101

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