Abstract
Living sensory neurons were studied by atomic force microscopy in the PeakForce QNM mode under near-physiological conditions. The dependence of the measured apparent Young modulus of cells on the ratio of the probe height to the beam length on a used cantilever was revealed. A qualitative explanation based on the analysis of beam strains in two limit cases, in which the probe slides over a studied object and the probe sticks to the latter, was given to the obtained result. It was proposed to classify native cells by the character of their interaction with the probe (sliding or sticking).
Similar content being viewed by others
References
T. G. Kuznetsova, M. N. Starodubtseva, N. I. Yegorenkov, S. A. Chizhik, and R. I. Zhdanov, Micron 38, 824 (2007).
M. Li, D. Dang, L. Liu, N. Xi, and Y. Wang, IEEE Trans. Nanobiosci. 16, 523 (2017).
C. Rotsch and M. Radmacher, Biophys. J. 78, 520 (2000).
M. Li, L. Q. Liu, N. Xi, and Y. C. Wang, Acta Pharmacol. Sin. 36, 769 (2015).
M.-Sh. Hung and M.-F. Tsai, Bionanoscience 5, 156 (2015).
A. V. Ankudinov, M. M. Khalisov, V. A. Penniyainen, S. A. Podzorova, and B. V. Krylov, Tech. Phys. 60, 1540 (2015).
M. M. Khalisov, V. A. Penniyainen, N. A. Esikova, A. V. Ankudinov, and B. V. Krylov, Tech. Phys. Lett. 43, 85 (2017).
E. Takai, K. D. Costa, A. Shaheen, C. T. Hung, and X. E. Guo, Ann. Biomed. Eng. 33, 963 (2005).
I. Sneddon, Int. J. Eng. Sci. 3, 47 (1965).
K. D. Costa and F. C. Yin, J. Biomech. Eng. 121, 462 (1999).
G. Meyer and N. M. Amer, Appl. Phys. Lett. 53 (12), 1045 (1988).
S. Fujisawa, M. Ohta, T. Konishi, Y. Sugawara, and S. Morita, Rev. Sci. Instrum. 65, 644 (1994).
H. Kawakatsu, H. Bleuler, T. Saito, and K. Hiroshi, Jpn. J. Appl. Phys. 34, 3400 (1995).
D. Sarid, Exploring Scanning Probe Microscopy with MATHEMATICA, 2nd ed. (Wiley-VCH, Weinheim, 2007), Chap. 2, p. 46.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.V. Ankudinov, M.M. Khalisov, V.A. Penniyaynen, S.A. Podzorova, K.I. Timoshchuk, B.V. Krylov, 2018, published in Pis’ma v Zhurnal Tekhnicheskoi Fiziki, 2018, Vol. 44, No. 15, pp. 38–45.
Rights and permissions
About this article
Cite this article
Ankudinov, A.V., Khalisov, M.M., Penniyaynen, V.A. et al. The Probe Length Effect on the Cantilever of an Atomic Force Microscope in Measuring the Mechanical Properties of Living Neurons. Tech. Phys. Lett. 44, 671–674 (2018). https://doi.org/10.1134/S1063785018080035
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063785018080035