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A microfluidic chip with hydrodynamic traps for in vitro microscopic investigations of single cells

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An Erratum to this article was published on 01 September 2016

Abstract

The results on making a microfluidic chip for in vitro microscopic investigations of single cells are presented. Numerical simulation of the motion trajectories of microparticles makes it possible to determine the geometry of hydrodynamic traps, their number, and the trap arrangement in a reaction chamber. According to the developed design, microfluidic chips were fabricated from a SU-8 photoresist by photolithography. The microfluidic chips have been tested to prove their operating capacity for isolating and holding K562 human myeloid leukemia cells from a sample flow and their subsequent investigation by confocal laser scanning microscopy.

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Authors and Affiliations

  1. ITMO University, St. Petersburg, Russia

    I. V. Kukhtevich, K. I. Belousov & A. A. Evstrapov

  2. Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, Russia

    I. V. Kukhtevich, A. S. Bukatin & A. A. Evstrapov

  3. St. Petersburg Academic University - Nanotechnology Research and Education Center, Russian Academy of Sciences, St. Petersburg, Russia

    A. S. Bukatin, M. V. Dubina & A. A. Evstrapov

Authors
  1. I. V. Kukhtevich
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  2. K. I. Belousov
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  3. A. S. Bukatin
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  4. M. V. Dubina
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  5. A. A. Evstrapov
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Corresponding author

Correspondence to A. S. Bukatin.

Additional information

Original Russian Text © I.V. Kukhtevich, K.I. Belousov, A.S. Bukatin, M.V. Dubina, A.A. Evstrapov, 2015, published in Pis’ma v Zhurnal Tekhnicheskoi Fiziki, 2015, Vol. 41, No. 5, pp. 103–110.

An erratum to this article can be found at http://dx.doi.org/10.1134/S1063785016090273.

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Kukhtevich, I.V., Belousov, K.I., Bukatin, A.S. et al. A microfluidic chip with hydrodynamic traps for in vitro microscopic investigations of single cells. Tech. Phys. Lett. 41, 255–258 (2015). https://doi.org/10.1134/S1063785015030086

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  • DOI: https://doi.org/10.1134/S1063785015030086

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