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Preparation of Water-Insoluble Silk Fibroin Films. Study of Their Structure and Properties

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Abstract

Conditions for obtaining water-insoluble silk fibroin in the form of films have been worked out. Using optical microscopy, a change in the surface morphology of the films was demonstrated due to a structural transition to a coarsely fragmented state as a result of the treatment of the films with methyl alcohol. The conformational transition of silk fibroin from the disordered structure of the untreated film to the β-folded structure was confirmed by the FTIR spectroscopy method. The glass-transition temperature of the native film equal to 216°C was determined by the method of differential scanning calorimetry. A study of the mechanical properties of silk fibroin films in a liquid medium showed that their strength decreases and elasticity increases by almost 15 times compared with tests of the same films in a dry state.

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Funding

This work was supported by the Russian Foundation for Basic Research, project no. 20-03-00400_a.

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Authors and Affiliations

  1. Federal State Budgetary Institution of Science Institute of Cytology of the Russian Academy of Sciences, 194064, St. Petersburg, Russia

    Yu. A. Nashchekina

  2. Peter the Great St. Petersburg Polytechnic University, 195251, St. Petersburg, Russia

    Yu. A. Nashchekina, V. S. Konygina, V. V. Kodolova-Chukhontseva & V. E. Yudin

  3. Institute of Macromolecular Compounds of the Russian Academy of Sciences, 199004, St. Petersburg, Russia

    E. N. Popova & V. E. Yudin

  4. Ioffe Institute, 194021, St. Petersburg, Russia

    Yu. A. Nashchekina & A. V. Nashchekin

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  1. Yu. A. Nashchekina
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  2. V. S. Konygina
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  4. V. V. Kodolova-Chukhontseva
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Correspondence to Yu. A. Nashchekina.

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Translated by V. Selikhanovich

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Nashchekina, Y.A., Konygina, V.S., Popova, E.N. et al. Preparation of Water-Insoluble Silk Fibroin Films. Study of Their Structure and Properties. Tech. Phys. 67, 297–303 (2022). https://doi.org/10.1134/S1063784222050073

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